Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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HighlightsMembrane protein antibodies play an crucial role in disease treatment and diagnosis due to their high specificity and sensitivity. However, the related technology is still not fully developed, mainly because of the unique characteristics of membrane proteins. One of the primary challenges in preparing membrane protein antibodies is the production of the membrane protein immunogen is the first difficulty. Creative Biolabs offers an advanced membrane protein research platform that can assist in the development of your membrane protein antibodies.
The Fab fragment, also referred to as the antigen-binding fragment, is a segment of the antibody structure responsible for binding to the antigen. It consists of a variable light chain (VL), a constant light chain (CL), a variable heavy chain (VH), and a constant heavy chain (CH1). Fab antibodies possess both antigen-binding and partially constant regions, giving them advantages such as comparable antibody-antigen affinity to single-chain antibodies, excellent tissue penetration, and more stable structure. These attributes make Fab antibodies vital in clinical diagnosis and therapy.
There are three main types of Fab fragment preparation precautions, which are enzymatic digestion, preparation using expression systems, and phage display techniques. Enzymatic methods employ proteases like papain and pepsin to degrade human immunoglobulin G and obtain F(ab')2, Fab, Fc fragments, and other products. The most commonly used expression systems for Fab antibody preparation are the E. coli expression system and mammalian expression system. In contrast, the Fab library method employs phage display technology to generate libraries of Fab fragment antibodies, which are subsequently obtained.
To screen Fab antibody fragments using Fab antibody libraries, the libraries are first created using phage display technology. After several rounds of screening and enrichment, high-affinity Fab antibodies can be obtained. Theoretically, Fab antibody libraries can yield a vast number of antibodies, reaching levels of 1010-1013. This significantly enhances the success rate of screening ideal antibodies.
The single-chain variable region (scFv) is an engineered form of the variable region. Unlike a disulfide bond, the scFv is created by joining two variable regions through a flexible junction. To create scFv, the intact antibody is artificially modified to express only the variable region. The heavy chain variable region of the antibody is then connected to the light chain variable region using a synthetic linker peptide gene, resulting in a recombinant gene. The antibody expressed by this gene is referred to as scFv. The preparation process of scFv gives it the characteristic of a small molecular weight. Additionally, scFv exhibits antigen-binding properties, high penetration power, a short in vivo half-life, and low immunogenicity for genetic engineering operations.
A commonly used method for scFv preparation is the utilization of phage display technology To construct scFv antibody libraries. These libraries involve the formation of numerous clones of the variable region (VH and VL) genes of antibodies, especially the complementary determining region (CDR). It uses different strategies and approaches, such as amino acid mutations and shifted reading frames to create diverse clones. Besides, it also involves the screening of high affinity scFv antibodies against the target antigen from these clones. The scFv antibodies with high affinity for the target antigen are screened from these clones. According to the origin of antibody genes, antibody libraries are generally classified into natural, immune, synthetic, and semi-synthetic antibodies.
At Creative Biolabs, we would like to share key insights and experiences in membrane protein antibody discovery and premade antibody libraries to facilitate the smooth running of your project.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
