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Magic™ Cell-free Membrane Protein Expression in E.coli Lysate - Dialysis Mode (CAT#: MPCFK-1-2)

Datasheet
Magic™ Cell-free Protein Expression in E.coli Lysate - Dialysis Mode Kit is a cell-free protein synthesis system using E.coli extracts. This system contains all the required components for protein synthesis and uses an efficient, coupled transcription/ translation reaction to produce proteins. The E.coli extract contains necessary components including ribosomes, protein translation factors, aminoacyl tRNA synthetase, total tRNA and others required for protein expression.
In a standard dialysis system, the user needs to mix the template DNA (plasmids or PCR products), E.coli extract, and other components into the dialysis system, and transfer it into a specific buffer. The outer solution contains amino acids, nucleotides, ATP and other factors. The inner solution contains all necessary components for E.coli cell-free protein expression. The dialysis will improve the in vitro synthesis yield.

Product Specifications

  • Lysate Type
  • E. coli Lysate
  • Size
  • 5 reactions
  • Reaction Volume
  • 200 µL per reaction
  • Expected Yield of Positive Control
  • 5-10 mg/ml
  • Components
  • Modified E. coli cell extract
    Modified reaction mix
    Outer mix
    Control template DNA
    Dilution buffer
    Dialysis system
    PCR1 Reaction mix
    PCR2 Reaction mix
    Z1 High Fidelity DNA Polymerase
    Z2 Ultra-Fidelity DNA Polymerase
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Get more protein

The operation of dialysis provides a synthetic yield of protein in vitro that can be used in experiments where protein demand is high.
2023.1.04

Q&As

How do you make hard-to-get protein synthesis easy?

There are 5 ways.
1.Cytotoxic Protein and Peptide Therapeutics.
2. Membrane Protein Synthesis.
3.CF Synthesis of Folded Proteins and Peptides.
4.Incorporation of NAAs into Proteins.
5.Post-Translational Modifications.
2023.01.06

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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