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Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate (CAT#: MPCFK-3-1)

Datasheet
Magic™ Cell-free Protein Expression in Wheat Germ lysate Kit is suitable for production of proteins with complicated conformation and multiple protein complexes, such as disulfide-bond-containing. This kit can ensure higher stability, yield and more flexibility for target proteins. This system contains all the required components for protein synthesis (ribosomes, translation initiation/elongation/enhancement factors, tRNA, polymerase, amino acids, ATP etc.). In Magic™ Cell-free Protein Expression in Wheat Germ lysate kit, protein expression reactions can easily be set up with few pipetting steps.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 50 reactions
  • Reaction Volume
  • 110 µL per reaction
  • Expected Yield of Positive Control
  • 0.1-0.2 mg/ml
  • Components
  • Wheat Germ Extracts
    Amino Acid Mix
    Transcription Reagent
    T7 RNA Polymerase
    25 mM NTP Mix
    100 mM DTT
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

High stability and protein activity

This kit can maintain the disulfide bond activity.
2023.1.08

Q&As

Is it possible to express functional Plasmodium falciparum enzymes using the wheat germ-free cell system?

Related studies show that the wheat germ cell-free system can express P.falciparum DHFR-TS protein, especially during construction or maintenance without any special effort to maintain the fully expressed plasmid under genetic or pharmacological stress.
2023.01.14

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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