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Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate - Bilayer Format (CAT#: MPCFK-3-3)

Datasheet
Magic™ Cell-free Protein Expression in Wheat Germ lysate - Bilayer Format Kit is a cell-free protein synthesis system using wheat germ extracts for protein production. This system contains all the required components for protein synthesis, so there is no need to prepare any reagents. The kit can be used either with plasmid DNA or with PCR DNA for protein expression.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 8 reactions
  • Reaction Volume
  • 226 µL per reaction
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • pEU-E01-MCS
    pEU-E01-DHFR(Positive Control)
    Wheat Germ Extracts
    Reaction buffer
    SPU PCR sense primer
    SP6 PCR sense primer
    Transcription Reagent
    Aluminum seal
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The kit contained complete components

There was no need to prepare other reagents, and it could be used with plasmids. The results are satisfactory.
2023.1.10

Q&As

How does the bilayer format cell-free expression system work?

The bilayer reaction is set up by first placing the reaction buffer into the reaction chamber. Because of the higher density of the wheat germ extract, the wheat germ reaction mixture can be easily pipetted below the reaction buffer to form two layers. During the diffusion controlled translation reaction the two layers will mix continuously supplying substrates for the translation reaction. Bilayer reactions can be customized by adding different additives to the reaction buffer in the upper layer (e.g. detergents, labeled amino acids) or the wheat germ reaction mixture in the lower layer (multiple RNA templates, liposomes) to offer special reaction conditions for protein labeling and the expression of membrane proteins.
2023.01.18

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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