MemDX™ DNA Transfection Reagent

Creative Biolabs proudly offers the specific MemDX™ reagent for efficient and safe DNA transfection. The research team, which includes a Nobel Laureate, also provides new classes of vector design. This MemDX™ DNA transfection reagent is applicable for various cell lines (e.g. epithelial, endothelial, fibroblast and myoblast cell lines) and different cell types (macrophages, monocytes, ES cells and primary cells).

Order Information

DESCRIPTION	CATALOG #	SIZE	PRICE
MemDX™ DNA Transfection Reagent-250	CBMD250	250 µL	Please Inquiry
MemDX™ DNA Transfection Reagent-500	CBMD500	500 µL	Please Inquiry

Description

MemDX™ DNA transfection reagent, which mainly based on a novel synthetic derivative from natural compounds, is particularly suitable for DNA delivery, and has shown outstanding transfection efficiency for both adherent cells and suspension cells. In addition, our MemDX™ DNA transfection reagent is also capable for the co-transfection of DNA and siRNA molecules, which enables gene expression and inhibition in the same experiment.

Key Characteristics

New supramolecular organization
High transfection efficiency
Ideal for primary and stem cells
Absence of toxicity at the effective concentrations
Excellent reproducibility
Using standard experimental conditions
Easy handling
Storage at 4 °C (± 2 °C)
No need to keep complexes on ice during transfection
No need to remove the transfection complex

Important Notes for Transfection

The complexes of MemDX™ DNA transfection reagent and DNA (or DNA and siRNA complex) must be prepared in medium that does not contain serum and antibiotics, but after the formation, the transfection can be achieved in the medium either with or without serum (depending on the cells).

Transfection conditions and expression level can be optimized according to the cell type, the used promoter, the property of expressed protein and the reagent and DNA amount.

Transfect in medium that does not contain serum and varying ratio among DNA quantity, reagent amount and cell density are helpful to obtain the highest transfection efficiency.

The MemDX™ DNA Transfection Reagents from Creative Biolabs are used for research purposes only, not intended for animal or human therapeutic or diagnostic use.

Product Data

Validated cell lines and cell types using MemDX™ DNA transfection reagent are shown as below:

Fibroblast Cell Lines	HEK293, COS-7, NIH3T3, Human Friboblasts, etc.
Epithelial Cell Lines	HeLa, CHO, MDCK, etc.
Endothelial Cell Lines	UVEC, etc.
Myoblast Cell Lines	C2C12, etc.
Neuronal Cell Lines	PC12 Rat Adrenal Gland Cells, etc.
Epithelial Cells	Caco2, etc.
Monocytes & Macrophages	RAW 264.7, etc.
ES Cells & Primary Cells	Murine Embryonic Stem Cells, Human Embryonic Stem Cells, Murine Blastocysts, etc.
Stem Cells	Rat Mesenchymal Stem Cells, Murine CD 34+ Stem Cells, etc.

MemDX™ DNA Transfection Reagent Fig. 1 Reporter gene expression following transfection via MemDX™ DNA transfection reagent. A. mouse embryonic stem cells, B. mouse mesenchymal stem cells, C. Chinese hamster ovary (CHO) cells, D. human hepatocellular liver carcinoma cells (HepG2).

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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