Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
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This product is a HEK293 stable cell line. It stably expresses tag-free AGTR1 receptor. When an agonist binds to AGTR1, G-protein signaling pathway is activated, leading to a cellular response mediated by calcium. Hence, the activity can be easily quantified on living cells in a dose-response manner by calcium concentration change. It can be applied in high-throughput drug screening and functional assays.
Product Information
Target Protein
AGTR1
Target Protein Species
Human
Accession Number
NM_000685
Protein Tag
Tag-free
Host Cell Type
HEK293
Target Classification
GPCR
Target Family
Angiotensin receptor
Target Research Area
Cardiovascular Research; Digestive and Renal Research; Infectious Research
Related Diseases
Renal Tubular Dysgenesis; Hypertension
Product Properties
Assay Types
Calcium flux assay
Resistance
G418
Stability
> 16 passages
Mycoplasma Testing
Negative
Biosafety Level
Level 1
Activity
Yes
Quantity
3x10⁶ cells
Form
Frozen cells
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
DMEM, 10% FBS, 300 mg/ml G418
Selective Antibiotic(s)
Regular antibiotics active against mycoplasmas, bacteria and fungi.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line. Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Incubation
37°C with 5% CO₂
Applications
Drug screening and biological assays
Application Notes
Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
Use Restrictions
These cells are distributed for research use only.
Shipping
Dry ice
Storage
Liquid nitrogen
Target
Full Name
Angiotensin II receptor type 1
Introduction
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants.
GPCR Signaling Pathway
The endogenous ligand is angiotensin II. Targeted protein activation can cause binding of Gq to G11 protein which, in turn, activate a phosphatidylinositol-calcium second messenger system.
Endogenous Ligand
Angiotensin II
Alternative Names
AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B; type-1B angiotensin II receptor; AGTR1; Angiotensin II receptor type 1