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Magic™ Human FLAG tagged HTR1A HEK293T Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX587)

Datasheet
This product is a HEK293T stable cell line. It stably expresses FLAG tagged HTR1A to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • HTR1A
  • Target Protein Species
  • Human
  • Accession Number
  • NM_000524.2
  • Protein Tag
  • FLAG-tag at N-terminus
  • Host Cell Type
  • HEK293T
  • Target Classification
  • GPCR
  • Target Family
  • Serotonin
  • Target Research Area
  • CNS Research
  • Related Diseases
  • Periodic Fever; Menstrual Cycle-Dependent; Generalized Anxiety Disorder

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Puromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS, 1 μg/mL puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • 5-hydroxytryptamine receptor 1A
  • Introduction
  • This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers.
  • GPCR Signaling Pathway
  • The endogenous ligand is serotonin. Targeted protein activation can cause binding of Gi to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.
  • Endogenous Ligand
  • Serotonin
  • Alternative Names
  • G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1;5-hydroxytryptamine receptor 1A;5-HT1a receptor;5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled;guanine nucleotide-binding regulatory protein-coupled receptor;Serotonin receptor 1A;HTR1A

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