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Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for GST-tagged Protein Production (CAT#: MPCFK-3-5)

Datasheet
Magic™ Small-scale Cell-free Protein Expression in Wheat Germ lysate for GST-tagged Protein Production Kit is a cell-free protein synthesis system using wheat germ extracts for GST-tagged protein production. The lysate is optimized and high-performance wheat germ extract and removed wheat proteins that would otherwise bind to glutathione during the purification step. This kit is premixed and allow to setup the transcription and translation reaction by just a few pipetting steps. It's a bilayer format for performing small-scale protein expression reactions. The kit can be used either with plasmid DNA or with PCR DNA for protein expression.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 16 reactions
  • Reaction Volume
  • 226 µL per reaction
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts
    Reaction buffer
    Transcription Reagent
    Aluminum seal
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Unnecessary impurities are avoided

The absence of wheat protein in the lysates did not affect the purification results.
2023.1.12

Q&As

What is the reaction format of the batch protein expression format of the wheat germ cell-free expression system?

In the batch method all reagents are mixed and incubated together for a limited period of time (commonly a few hours).
2023.01.22

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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