Does Creative Biolabs support custom or non-natural PTM types provided by the client (e.g., phosphorylation mimics, citrullination, sulfation)?

Yes. Any chemically synthesized or enzymatically prepared PTM can be used as bait, provided its structure is defined. The team will assess presentation stability on the yeast surface or in solution phase. A preliminary feasibility trial is suggested for unnatural modification projects.

Can the platform identify proteins or antibodies that recognize cross-regulatory interactions between multiple PTMs on the same target (e.g., both phosphorylation and ubiquitination at adjacent sites)?

Absolutely. We could perform screening against differentially modified peptides (single vs. dual PTMs) with counter-selection against each individual modification. This allows isolation of clones that require the simultaneous presence of two PTMs - a powerful tool for studying PTM crosstalk in signaling hubs.

Does Creative Biolabs perform downstream validation or orthogonal assays after hit identification?

Yes. The service can be extended to include recombinant expression of selected clones (antibodies or other binding proteins) followed by orthogonal validation: co-immunoprecipitation from cell lysates, GST/His pull-down with purified PTM proteins, or surface plasmon resonance (SPR) for affinity and specificity kinetics. These are quoted separately.

Yeast Display based Post-Translational Modification (PTM) dependent Protein Interaction Identification Service

Services Workflow Platform Advantages Related Services FAQ

PTM-Dependent Protein Interaction Screening

Post-translational modifications (PTMs) dynamically govern protein interaction networks, yet mapping PTM-dependent protein interactions poses significant technical hurdles. Creative Biolabs provides dedicated yeast display screening services to capture protein interactions that rely exclusively on specific PTM profiles. Our portfolio includes phosphorylated peptides, ubiquitinated lysine residues, acetylated amino acids, small-molecule signaling ligands, and additional biomolecular substrates. We also enable the discovery of monoclonal antibody fragments capable of distinguishing modified and unmodified PTM epitopes.

Consult Our Technical Experts →

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Discover high-affinity anti-GPCR specific antibodies.

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pH-dependent Antibody Screening Service by Yeast Display

Select antibodies with pH-responsive binding property.

Obtain Custom Technical Quotation →

Workflow - Transparent, Controllable, and Trustworthy

Starting Materials
Client-provided synthetic modified peptide, recombinant protein bearing defined PTM, or a PTM-enriched cellular lysate. Alternatively, only the target protein sequence and modification site information.

Key Steps

Library construction

Amplification of yeast display library (naïve, immune, or synthetic) with diversity covering PTM-recognition domains.

Library Screening

Sequential rounds of magnetic and fluorescence-activated sorting against the PTM-specific bait.

Counter-selection

Depletion of clones that bind the unmodified counterpart to ensure strict PTM dependence.

Hit Identification

Single-clone isolation, plasmid recovery, and sequencing.

Validation

Re-display of selected clones to confirm PTM-dependent binding by flow cytometry.

Contact Our Scientists to learn more about process!

Platform - Highlight Core Technology Strength

The yeast display platform offers unique advantages for PTM-dependent interaction discovery:

Eukaryotic
Folding

FACS

Quality
Control

PTM
Compatibility

Eukaryotic folding machinery
Preserves conformational epitopes and authentic PTM-induced structural changes.

High-throughput sorting (FACS)
Enables quantitative discrimination of binding affinity against modified vs. unmodified targets.

Quality control by surface density normalization
Eliminates expression-level artifacts during PTM-specificity sorting.

Compatible with a wide range of PTMs
Phosphate, acetyl, ubiquitin, SUMO, methyl, and custom chemical modifications.

Feel free to contact us to know more!

Why Choose Us - Differentiators & Evidence

Fig.9 Researcher (Creative Biolabs AI)

Management Advantages
Dedicated PhD team for project docking. Expertise in PTM biology and protein engineering with one-on-one full-cycle technical support.

Fig.10 Experiment (Creative Biolabs AI)

Resource Advantages
Large-scale human proteome library with diverse protein modification sites; custom substrate screening available.

Fig.11 Structure (Creative Biolabs AI)

Technical Advantages
Shorter lead time & higher cost performance. Higher efficiency than conventional phage display with transparent pricing.

Fig.12 Plan (Creative Biolabs AI)

Team Advantages
Proven experience in downstream applications with successful cases across various fields.

Contact our technical team to discuss your experiment details →

Broaden Your Research Scope & Improve Project Conversion

Explore upstream and complementary offerings to build a complete discovery pipeline:

Fig.13 Grid (Creative Biolabs AI)

Answers to Questions

Does Creative Biolabs support custom or non-natural PTM types provided by the client (e.g., phosphorylation mimics, citrullination, sulfation)?
Yes. Any chemically synthesized or enzymatically prepared PTM can be used as bait, provided its structure is defined. The team will assess presentation stability on the yeast surface or in solution phase. A preliminary feasibility trial is suggested for unnatural modification projects.
Can the platform identify proteins or antibodies that recognize cross-regulatory interactions between multiple PTMs on the same target (e.g., both phosphorylation and ubiquitination at adjacent sites)?
Absolutely. We could perform screening against differentially modified peptides (single vs. dual PTMs) with counter-selection against each individual modification. This allows isolation of clones that require the simultaneous presence of two PTMs - a powerful tool for studying PTM crosstalk in signaling hubs.
Does Creative Biolabs perform downstream validation or orthogonal assays after hit identification?
Yes. The service can be extended to include recombinant expression of selected clones (antibodies or other binding proteins) followed by orthogonal validation: co-immunoprecipitation from cell lysates, GST/His pull-down with purified PTM proteins, or surface plasmon resonance (SPR) for affinity and specificity kinetics. These are quoted separately.

Receive tailored proposals aligned with your research goals!

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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