Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights
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The 298-amino acids-type I transmembraneglycoprotein Junctional adhesion molecule 2 (JAM2) is an immunoglobulin superfamily member. JAM2 expression within high endothelial venules (HEVs) in tonsil and lymph nodes, as well as additional expression on the endothelium of arterioles in and around inflammatory and tumor foci are well-characterized niches for JAM2. Whereas JAM-A is expressed on endothelial and epithelial molecule, as well as leukocytes and platelets; the expression of JAM2 is predominantly restricted to endothelial cells, certain subsets of leukocytes. It functions as an adhesive ligand mediating many other immune cell types T cells, NK cells, dendritic cells and monocytes and its also acts as a counterreceptor to the general leukocyte integrin VLA-4 (α4β1, CD49d/CD29) facilitating rolling adhesion firm adhesion, transendothelial migration of leukocytes.
Fig.1 The basic structure of JAMs.1
JAM2 contributes to the maintenance of endothelial barrier, leukocyte trafficking, and homeostasis of neurovascular units:
Creative Biolabs provides high-quality JAM2 membrane protein products to support structural and functional studies of this endothelial junctional adhesion molecule. Our membrane protein platform provides JAM2 constructs and preparations suitable for a range of research applications, including structural studies, ELISA, antibody production and characterization, binding assays, and investigations of JAM2-related cell adhesion and junctional biology. Available products may include membrane-associated JAM2 proteins or extracellular-domain constructs for studies of JAM2 interactions with relevant adhesion and signaling partners. Product format, expression system, and functional characteristics should be determined according to the corresponding product information and intended research application.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Creative Biolabs's stable cell lines provide a robust, reproducible platform for JAM2 biology and pharmacology in drug screening including; leukocyte adhesion assays, transendothelial migration analyses, tight junction permeability assays and high-throughput-screening. Our experience guarantees ideal expression and cell safety becomes more advanced quickly. These include JAM2-overexpressing lines to characterise the binding properties of JAM-C and the functional assays for VLA-4-mediated adhesion, whilst two JAM2-knockdown lines are included in these studies for loss-of-function analyses (see summary of validated expression as by flow cytometry, Western blotting and functional leukocyte binding assays). TEER (transendothelial electrical resistance) sensors or paracellular tracer permeability readouts can be used in specialized reporter cell lines for real-time measures of tight junction integrity and strongly facilitate therapeutic screening at different models.
Not finding the stable cell line product you need? Contact us to start your one-stop custom service!
We have developed a portfolio of recombinant antibodies targeting JAM2 for a range of research applications. Recombinant production supports sequence-defined, renewable reagents with consistent lot-to-lot production. Depending on the specific antibody and supporting datasheet, selected JAM2 antibodies may be suitable or available for applications such as Western Blotting (WB), ELISA, Flow Cytometry (FCM), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), and Immunoprecipitation (IP). These antibodies can support the detection and analysis of JAM2 in appropriate research samples and model systems. Product-specific epitope information, species reactivity, sample compatibility, specificity, and application performance should be determined according to the validation data available for each individual antibody.
Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!
In addition to our catalog products, Creative Biolabs specialize in custom services of membrane protein and antibody discovery & development. Drawing on our broad head and world class specs platforms, we may assist you:
Yes, we supply antibodies that are selective for the conformation of an epitope contained within a membrane-proximal C2 domain exposed in cis-dimerization and trans-interaction activated by JAM3 at cell-cell junctions. The above-mentioned reagents allow for direct immunofluorescence visualization of the engaged, junctionally localized tissue JAM2 population in fixed endothelial monolayers or tissue sections, which gives a readout of functional adhesive complex formation independent of paracellular permeability or transepithelial electrical resistance instrumentation.
Yes, dual-stable lines licitarily express both full-length JAM2 or—JAM3 from independent loci in and an endothelial compatible background. Utilizing a Finalmap, each batch is released with quantified measures of junctional strand density, physiological lymphocyte adhesion and transmigration efficiency that serves as minimally-disruptive cellular standard for reproducible mechanistic and pharmacological studies without the need for re-iterated transient transfection.
Yes, selected clones were validated on archival specimens after antigen retrieval and show specific junctional staining patterns at endothelial cell borders co-localizing with established tight junction markers ZO-1 and occludin.
Yes, the protein is concentrated in a defined endotoxin-monitored buffer at physiological pH, devoid of carrier proteins and denaturing agents. This formulation is compatible with direct dilution into bio-layer interferometry reactions, coupling of lysates to microbead surfaces for lymphocyte adhesion assays or incorporation within supported lipid bilayers, such as required for junctional reconstitution without the need for dialysis and/or buffer exchange.
