Close
Close
Loading...
CONTACT US
:
:
:
Call us at:
:
:
:
Fax:
Email:

PSENEN

Products

Loading...

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

Presenilin enhancer, gamma-secretase subunit (PSENEN), also known as PEN-2, is a small integral membrane protein of approximately 101 amino acids and an essential component of the γ-secretase complex. Together with presenilin, nicastrin, and APH1, PSENEN contributes to the assembly and maturation of the functional γ-secretase complex. Presenilin contains the catalytic aspartate residues responsible for intramembrane proteolysis, whereas PSENEN plays an important role in stabilizing the complex and promoting presenilin endoproteolysis into its N-terminal and C-terminal fragments. Loss or disruption of PSENEN can impair γ-secretase complex maturation and reduce γ-secretase-mediated substrate processing.

Fig.1 Differential expression of PSENEN in normal and tumor samples from patients with 31 types of cancers. (OA Literature)Fig.1 Gene expression and protein information of PSENEN.1

PSENEN Protein Function: A Gatekeeper of Gamma-Secretase Assembly and Activity

PSENEN is involved in multiple interconnected biological processes:

  • Presenilin Maturation and Complex Stabilization: PSENEN binds the transmembrane domains of presenilin holoprotein, inducing autocatalytic cleavage into NTFs (N-terminus fragments) and CTFs (C-terminal fragments), both forms noncovalently associated. The endoproteolysis event is the key activation step for this protease.
  • Notch Signaling and Developmental Fate Determination: One of the crucial steps in determining cellular fates is through regulation via the cell membrane by signalling pathways such as those involving Deltas/ligands (DSL ligands), their cognate receptors, known as Notches (N) that require γ-secretase function to drive transcription programs. Embryonic deletion of Psenen in mice gives rise to a severe Notch-deficiency phenotype, highlighting the non-redundant role for this subunit in developmental signaling. PSENEN controls apical progenitor transition to neurons and oligodendrocyte precursor differentiation in the developing neocortex and hippocampus.
  • Disease Associations: The reported loss of function for PSENEN is in keeping with the observation that haploinsufficiency at 19q13 causes familial acne inversa-2 (ACNINV2) a chronic inflammatory skin disorder and lends further evidence to its pathophysiologic role as suggested by Dowling-Degos disease, both on the basis of our findings. Defective Notch signalling in urban and melanocytes also underlie these conditions, showing that there are widespread non-CNS gamma-secretase physiological roles.

PSENEN Membrane Protein Product

Explore our recombinant PSENEN protein products for structural and functional studies of the γ-secretase complex. Because of its compact size, multipass membrane topology, and interaction with other γ-secretase components, PSENEN presents specific considerations for recombinant protein design and preparation. Our portfolio may include full-length wild-type PSENEN and engineered research constructs suitable for structural studies, protein-interaction research, γ-secretase complex-related investigations, and antibody development and characterization. Available expression formats, sequence variants, membrane-associated preparations, and functional characterization should be determined according to the corresponding product information and supporting data.

PSENEN Protein Product

Not finding the membrane protein product you need? Contact us to start your one-stop custom service!

PSENEN Stable Cell Line Product

Our stable cell lines have been developed expressing wild-type or disease-linked forms of mouse and rat PSENEN along with modulated endogenous expression for loss-of-function analysis. These suites are designed with regard to high-throughput gamma-secretase inhibitor screening, APP processing assays, Notch reporter gene analysis, and complex assembly quantification, providing the consistency in experimentation required for multi-phase research projects.

PSENEN Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

PSENEN Recombinant Antibody Product

We focus on research and development of high-affinity recombinant antibodies targeting PSENEN, to meet your rigorous requirements for quantitative detection in many applications. Carefully developed and manufactured only through advanced recombinant technologies, these antibodies demonstrated superior specificity, sensitivity, consistency between batches etc. in comparison to traditional polyclonal ones. PSENEN recombinant antibodies have been validated in WB, ELISA,FCM, IF,ICC and IHC. There are vast binding regions which can be used to detect PSENEN from different sample types like membrane preparations (membrane component), tissue lysates or reconstituted gamma-secretase complexes.

PSENEN Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Superior Batch-to-Batch Consistency: Stringent quality controls to ensure low variability from batch to batch.
  • Customization Options: Tags/Labels/package Modifications available upon request.

Custom PSENEN Membrane Protein and Antibody Discovery Services

Aside from our catalog products, we provide tailored custom services for discovery and development of membrane proteins and antibodies:

  • Production & Validation: Cloning of the synthesized optimized gene into an expression vector, plus small-scale expression trials to help determine the best expression conditions.
  • Custom Antibody Production: Produced via large-scale expression in mammalian/insect/cell culture or bacterial expression systems, purified in detergent or reconstituted into nanodiscs.
  • Engineered Cell Lines: Creation of stable cell lines that physiologically mimic endogenous PSENEN expression and activity.
  • Functional Assessment Systems: Designing and executing assays to assess protein function and ligand binding.

Frequently Asked Questions (FAQ)

  1. Are your PSENEN products suitable for clinical or diagnostic use?

    No, our PSENEN products and services are strictly for research use only.

  2. What types of PSENEN products do you offer?

    We offer a recombinant full-length PSENEN, stable cell lines which express related proteins including PSEN1 or gamma-secretase components, animal products, and high-quality recombinant antibodies against the protein of interest as well custom services.

  3. Do you offer stable cell lines with inducible PSENEN expression or co-expression with presenilin?

    Absolutely. Our cell engineering team routinely generates tetracycline-inducible systems and lines co-expressing PSENEN with PSEN1, PSEN2, NCSTN, or APH1 for comprehensive gamma-secretase reconstitution studies. We welcome detailed discussions regarding your preferred expression architecture and phenotypic requirements.

  4. Can I request bulk quantities or custom formulations for high-throughput screening?

    Yes. We accommodate academic and industrial scale-up requirements with flexible pricing tiers. Please submit your projected volume and timeline through our inquiry portal to receive a tailored quotation.

Reference
  1. Chen, Kaijie, et al. "Immunological and prognostic analysis of PSENEN in low-grade gliomas: An immune infiltration-related prognostic biomarker." Frontiers in Molecular Neuroscience 15 (2022): 933855. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fnmol.2022.933855
Our customer service representatives are available 24 hours a day, 7 days a week. Contact Us
© 2026 Creative Biolabs. | Contact Us
;