Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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Transmembrane protein 175 (TMEM175) is a multi-pass transmembrane protein encoded by the TMEM175 gene, composed of multiple transmembrane helical segments and cytoplasmic terminal domains, and acts as a core lysosomal cation channel subunit for endolysosomal ion equilibrium complexes. TMEM175 is widely expressed across lysosome-rich parenchymal, secretory and stromal cell populations, serving as a core scaffold mediating intralysosomal cation balance and downstream autophagic kinase recruitment upon nutrient and stress stimulation.
TMEM175 exerts its biological effects through sustained ion-permeable pore assembly within endolysosomal membranes, a mechanism that modulates intralysosomal cation gradient stability and intracellular autophagic signal cascade activation efficiency. Unlike substrate-specific lysosomal transporters, TMEM175 cannot independently drive complete autophagic flux and relies on intact channel conformation to sustain full signal output; it recruits cytoplasmic signal mediators to lysosomal membrane complexes, uncoupling luminal ion homeostasis regulation from intracellular autophagy transcription factor activation and sustaining persistent cellular stress response signals. This dual regulation modulates the intensity of lysosomal catabolic responses after metabolic stimulation, fine-tuning local cellular degradative activity and autophagy mediator secretion levels, while sustained abnormal TMEM175-mediated ion conduction drives impaired lysosomal clearance and degenerative tissue lesions. TMEM175 participates in key physiological and pathological processes including lysosomal acid-base balance, autophagosome maturation, metabolite-mediated cellular stress and chronic degenerative disorders. Dysregulation of TMEM175 expression or channel permeation capacity is closely associated with disrupted lysosomal function and impaired cellular homeostatic defense, making TMEM175 a crucial research target for lysosomal ion signaling, autophagy and degenerative disease research.
Fig. 1 Topological and structural comparison of prokaryotic and eukaryotic TMEM175. (A) Schematic topology of prokaryotic TMEM175 with four 6-transmembrane (6TM) repeats. (B) 3D structural model of prokaryotic TMEM175. (C) Linear transmembrane topology of eukaryotic TMEM175 consisting of two tandem Repeat units (each with two 6TM segments). (D) Cryo-EM resolved 3D structure of human TMEM175 lysosomal cation channel.1
The biological functions of TMEM175 are focused on endolysosomal ion channel assembly, autophagic signal propagation and cellular metabolic balance:
Creative Biolabs offers high-purity TMEM175 proteins through optimized heterologous expression systems, including full-length TMEM175 and isolated transmembrane domain variants with modified glycosylation status. These products retain native conformational characteristics, suitable for lysosomal ion transport research, membrane complex interaction detection, and small molecule autophagy modulator screening for degenerative disorder research. All TMEM175 proteins undergo strict quality control, including purity analysis and biological activity validation to ensure biological function.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Creative Biolabs provides custom-engineered TMEM175 stable cell lines, including overexpressing and knockdown models in parenchymal and secretory cell models. These cell lines are optimized for studying TMEM175-mediated lysosomal channel assembly mechanisms, autophagic signal cascade dynamics, and autophagy regulatory compound sensitivity. Each cell line undergoes stringent validation, including stable expression detection and functional integrity verification.
Not finding the stable cell line product you need? Contact us to start your one-stop custom service!
High-specificity recombinant antibodies targeting TMEM175 are developed via advanced antibody engineering technologies. These antibodies are validated for multiple applications, including immunofluorescence for TMEM175 lysosomal membrane localization, Western blot for expression analysis, and co-immunoprecipitation for TMEM175-membrane complex research, enabling precise analysis of TMEM175 expression, subcellular localization and functional regulation.
Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!
Beyond catalog products, Creative Biolabs offers specialized custom services for TMEM175 research:
TMEM175 is a lysosomal transmembrane cation channel that forms functional membrane pores to modulate intralysosomal cation gradients, adjust cellular autophagic response dynamics, and mediate lysosomal degradative activation under metabolic stress stimulation.
TMEM175 is a core regulator of lysosomal ion homeostasis and cellular autophagic signaling, and its dysregulation is associated with impaired lysosomal clearance and chronic degenerative lesions. It is a critical target for lysosomal transport and autophagy research.
No, all TMEM175 products and services are strictly for research use only, not intended for clinical diagnosis or treatment.
Offerings include TMEM175 proteins (full-length, transmembrane domain variants), specific recombinant antibodies, and custom stable parenchymal cell lines, supporting lysosomal transport and degenerative disorder research.
TMEM175 proteins are validated by lysosomal membrane complex binding assays and autophagic signal regulation verification to ensure native regulatory function in lysosomal research.
