Single Domain Antibody (SdAb) Discovery Service by Yeast Display

Services Workflow Features Related Services Introduction

SdAb Discovery and Evaluation by Yeast Display Platform

Using our comprehensive yeast display platform, we can reliably and rapidly discover high-affinity single-domain antibodies (sdAbs), and we can also customize and optimize these antibodies to make them suitable for a variety of drug therapies, such as ADC and CAR-T.

Fig 1. A picture demonstrating sdAb. (Creative Biolabs Authorized)

Premade Libraries Available to Accelerate sdAb Development!

We simplify the sdAb development process by offering access to both naturally derived libraries and premade sdAb libraries, streamlining antibody selection for your specific needs. Our scientific experts provide swift and accurate outcomes along with expert guidance throughout every step of the process.

Fig 2. A graph demonstrating camelids. (Creative Biolabs AI)

Immune or Synthetic SdAb Library Construction for Your Custom Selection

Our comprehensive immune library-based service initiates with camelid immunization (llamas, alpacas, camels), which leads to the production of small and stable single-domain antibodies. We integrate the VHH genes into a yeast display system to generate a diverse antibody library that targets your specific antigens.

High-Throughput Screening

Our high-throughput screening method allows us to pinpoint sdAb that demonstrate superior binding affinity and specificity. After identification, sdAb could be selected for further iterative engineering for affinity maturation, which improves their binding strength and stability. The resulting optimized sdAbs are produced using tailored systems, suitable for a variety of applications. Our experts deliver steady support throughout each step to make sure the solutions match your project requirements precisely.

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Workflow for SdAb Discovery and Evaluation

Initial Consultation and Requirement Finalization

Initial discussions with the client to clarify project goals, requirements, and specific antibody needs (e.g., antigen types).
Finalize the experimental design and timeline.

Sample Preparation

Antigen preparation and labeling.
Starting materials for library construction or screening.

Custom Library Design and Construction

Design and generate a tailored sdAb library according to the client’s specifications, utilizing immune samples or synthesized sequences.
Directly select our premade sdAb libraries for further screening.

Library Screening and Binder Validation

Conduct high-throughput screening using the yeast display platform to identify initial binder candidates that bind to the targets.

Binding and Functional Evaluation

Perform in-depth binding analysis and functional evaluation of the selected candidate antibodies, ensuring they exhibit the desired affinity and biological activity.

Affinity Optimization

Optimize the affinity and specificity of the antibodies, improving their binding strength to the targets.

Format Conversion and Engineering

Convert the optimized antibodies into the required format (e.g., Fc fusion proteins, bispecific, and multivalent constructs) and perform necessary engineering modifications to enhance their performance and stability in various applications.

Why Choose Yeast Display for SdAb Discovery?

High-Throughput Screening

Rapidly screens large libraries to identify high-affinity sdAbs, accelerating the discovery process.

Optimization of Binding Properties

Allow for affinity maturation, improving specificity and binding strength.

High Specificity and Affinity

Enable discovery of sdAbs with exceptional specificity, even for challenging targets like cryptic epitopes.

Direct Display on Yeast Surface

SdAbs displayed on yeast cells for real-time binding analysis, simplifying the identification of binders.

Robust and Efficient Process

Deliver reliable results quickly, optimizing your R&D timeline.

Tailored Solutions

Customizable workflows to meet specific project requirements.

Minimal Cross-Reactivity

Select sdAbs with high specificity, reducing non-specific binding.

Expert Support

Continuous support from an experienced team throughout the discovery and optimization process.

Related Services

Creative Biolabs delivers supporting complementary services for your antibody development projects which extend beyond the following options:

What Are SdAbs?

The unique class of antibody fragments known as sdAbs or VHHs stands out due to their small size and exceptional stability. sdAbs differ from traditional antibodies because they consist of a single variable domain either naturally derived from camelid antibodies or through synthetic engineering to replicate this basic structure.

SdAb domains maintain high specificity binding capabilities across numerous targets, including unreachable epitopes for conventional antibodies like hidden or conformational epitopes. The wide temperature stability and minimal immunogenicity of sdAbs enhance their applicability in both research laboratories and clinical environments. Researchers can modify sdAbs to display customized binding properties which make them adaptable instruments for various uses including drug discovery and medical imaging.

Get in Touch with Us

Reach out to us today to discover our pioneering antibody discovery platforms which can help realize your project goals. We look forward to collaborating with you!

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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