Creative Biolabs has developed Single Protein Production (SPP)™ technology for high-throughput protein production. In the SPP system, live E. coli cells are converted into a bioreactor producing only a single protein of interest in a high yield. A yield of 20–30% of total cellular protein can be obtained with our technology, which overwhelms all protein production methods known so far.
With the evolution of our technology, we are now capable to produce any proteins with the SPP system. With the technology, protein yields can be kept unaffected even when the culture is condensed up to 40-fold, and this reduces the cost of protein production by up to 97.5%. The technology provides isotope-labeled proteins at a very high signal-to-noise ratio. More than 90% of the isotope can be incorporated into the target protein in the SPP system. Furthermore, a refinement of our technology has eliminated lengthy purification steps in the production of membrane proteins suitable for structural studies.