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Insect Cell based Protein Expression Service

Creative Biolabs' Baculovirus/ Insect Cell Expression Platform was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells in a flexible scale.

Baculovirus/ Insect Cell Expression System

The baculovirus expression vector system (BEVS) is universally recognized as a powerful and versatile system for the production of high-quality proteins. There are several advantages of insect cells over E. coli such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins.

Workflow of Our Insect Cell based Protein Expression Service

The gene encoding the protein of interest is cloned into a suitable transfer vector, such as pFastBac1. This vector contains elements necessary for the expression of the gene in insect cells. The transfer vector is co-transfected with baculovirus DNA into insect host cells to obtain recombinant baculoviruses containing the gene of interest.

The recombinant baculovirus is amplified through multiple rounds of infection and harvesting to ensure that a sufficient quantity of recombinant virus is available to infect insect cells at a large scale.

The expressed protein is purified using affinity chromatography. The choice of purification method depends on the protein's properties and the presence of any affinity tags that were included during gene cloning.

The final product undergoes quality control tests to verify its purity, activity, and structural integrity. We can provide quality control methods including SDS-PAGE, WB, ELISA, and so on.

For customers who wish to establish the best possible protocol for their project and then scale up, Creative Biolabs recommends customers leverage sophisticated platforms to explore different conditions for the expression of target proteins. If you have any questions about our service, please contact us.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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