Introduction of ABCA8
ABCA8 encoded by ABCA8 gene is a member of the superfamily of ATP-binding cassette (ABC) transporters which transport various molecules across extra- and intracellular membranes. There are seven subfamilies in ABC superfamily named as ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White, respectively. ABCA8 protein with a classical full-transporter structure belongs to ABC1 subfamily. It is predicted to have 14 membrane-spanning segments and two ATP-binding sites.
|Basic Information of ABCA8|
|Protein Name||ATP-binding cassette sub-family A member 8|
|Organism||Homo sapiens (Human)|
Function of ABCA8 Membrane Protein
ABCA8 is a little-known member of ABC1 family which transport lipids across cellular membranes. It is reported that ABCA8 is expressed in multiple regions of adult human brains with a spatial specificity. The ABCA8 expression in oligodendrocyte-enriched white matter regions is significantly higher than the grey matter cortical regions. Further study shows that ABCA8 in oligodendrocytes can significantly promote the of sphingomyelin synthase 1 expression and increase sphingomyelin production. Moreover, the ABCA8 expression in the prefrontal cortex is strongly associated with age-associated myelination and the myelinating gene p25α is significantly increased with ABCA8. Besides, the aberrant ABCA8 expression may play a role in the early pathogenesis of multiple system atrophy (MSA), a fatal neurodegenerative disease, through influencing myelin stability and regulation of synuclein and p25. In addition, it has been revealed that ABCA8 functions as an ATPase-dependent drug transport protein. Its specific substrates are very close to the multidrug resistance-associated protein 2 (MRP2; ABCC2) such as [14C] estradiol-β-glucuronide, taurocholate, and LTC4, and organic anion transporter (OAT), such as para-aminohippuric acid, ochratoxin-A.
Fig.1 Visual overview of cholesterol transport and high-density lipoprotein cholesterol formation. (Triguerosmotos, 2017)
Application of ABCA8 Membrane Protein in Literature
The study indicates that ABCA8 serves as a sinusoidal efflux transporter for at least taurocholate and cholesterol in human liver and mouse.
The study shows that ABCA8 plays an essential role in the regulation of cholesterol efflux and high-density lipoprotein cholesterol levels.
The study proposes a valuable tool for classifying breast fibroepithelial lesions into fibroadenomas and phyllodes tumors by analyzing five-gene transcript set (ABCA8, APOD, CCL19, FN1, and PRAME).
The study reveals that the expression of ABCA8, ABCB1, ABCC6, ABCC9, ABCC10, ABCD2, ABCG2, and ABCG4 is significantly decreased in prostate cancer (PCa) compared to noncancerous prostate tissues (NPT).
The study first identifies the protein expressions of some transporters such as MATE1 and ABCA8 in the plasma membrane fractions of rat and human choroid plexuses.
ABCA8 Preparation Options
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