Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsAt Creative Biolabs, we leverage two decades of expertise to provide an industry-leading Antibody Repertoire Analysis Solution, utilizing high-resolution sequencing and AI-integrated bioinformatics to transform complex biological samples into actionable therapeutic data. Our Antibody Repertoire Profiling platform is designed for researchers who demand the highest level of accuracy, depth, and physiological relevance. By bypassing traditional hybridoma limitations, we offer a direct window into the functional immune landscape, enabling the discovery of rare, high-affinity antibodies and the characterization of complex peptide–MHC complexes interactions.
The ability to map the immune repertoire has transformed from a fundamental research tool into a strategic necessity across the pharmaceutical value chain. Whether the goal is to develop a novel checkpoint inhibitor or to evaluate the long-term memory induced by a vaccine, Antibody Repertoire Sequencing (Rep-Seq) provides the quantitative framework needed for success.
Fig. 1 Paired AIRR sequencing of the pro-B, pre-B, immature and naïve heavy, kappa and lambda Ab repertoire and long-read genomic sequencing of the immunoglobulin loci.1
The MemDX™ Antibody Repertoire Analysis platform is built on a foundation of proprietary molecular biology protocols and advanced computational algorithms. Unlike standard sequencing services, MemDX™ focuses on maintaining the biological context of every sequence, ensuring that the data generated is as functionally relevant as it is deep.
| Capability | Technical Detail | Strategic Value |
| High-Resolution Resolution | Individual cell-level partitioning and barcoding | Native H/L andα/β pairing |
| Ultra-Deep Rep-Seq | Up to 109 reads using optimized NGS pipelines | Comprehensive coverage of the total repertoire diversity. |
| 5' RACE Integration | Template-switching oligo-based amplification | Unbiased capture of the entire V(D)J region, including unknown V-genes. |
| UMI Error Correction | Unique Molecular Identifiers for every mRNA | Elimination of PCR bias and 99.9% reduction in sequencing artifacts. |
| pMHC Targeting | Specialized enrichment for peptide–MHC complexes | Direct discovery of antigen-specific TCRs and TCR-like antibodies. |
Our bulk sequencing service is the gold standard for population-level Antibody Repertoire Profiling. We have moved beyond the limitations of multiplex PCR, which often fails to amplify certain V-gene families due to primer mismatching.
One of the primary challenges in Antibody Repertoire Analysis is the loss of heavy/light chain pairing information during standard bulk processing. MemDX™ solves this through a proprietary micro-compartmentalization approach. By partitioning immune cells into individual reaction environments—such as micro-droplets or nanowells—we can physically isolate the mRNA of a single cell. Within these environments, a unique barcode is attached to both the heavy and light chain transcripts. When the samples are pooled and sequenced, our bioinformatics pipeline uses these barcodes to reconstruct the original, biologically functional pair.
In the realm of T-cell therapy, the interaction between the TCR and the peptide–MHC complexes is the "holy grail". MemDX™ incorporates specialized antigen-binding assays that utilize pMHC multimers. We can selectively enrich for T cells that bind to a specific pMHC target before performing Antibody Repertoire Sequencing. This ensures that the resulting data is not just a list of sequences, but a functional map of antigen-specific immunity. This is particularly valuable for:
The true power of the MemDX™ Antibody Repertoire Solution lies in our computational core. Raw sequencing data is processed through a multi-stage AI-driven pipeline designed for maximum accuracy.
The first stage involves the identification and consolidation of Unique Molecular Identifiers (UMIs). By grouping reads that share the same UMI, we can distinguish between a clone that is truly expanded in the patient and a sequence that was simply over-amplified during PCR. This provides an absolute quantitative count of the immune repertoire.
Using the most comprehensive versions of databases, MemDX™ identifies the V, D, and J segments of every sequence. We calculate the level of somatic hypermutation (SHM) by comparing the observed sequence to the inferred germline, allowing us to quantify the "age" and maturity of the immune response.
The CDR3 region is the primary determinant of antigen binding. Our Antibody Repertoire Profiling includes:
We build sophisticated phylogenetic trees that show the evolutionary trajectory of B-cell clones. This is essential for understanding how an antibody evolves from a low-affinity germline precursor to a high-affinity therapeutic candidate.
For pharmaceutical companies, MemDX™ serves as a high-speed discovery engine. By sequencing the repertoires of immunized animals (including transgenic "humanized" mice or camelids), we can identify thousands of potential leads. This "Next-Gen Discovery" eliminates the bottleneck of traditional screening and provides a much broader pool of candidates from which to select for developability and affinity.
A successful vaccine must do more than produce a high titer of antibodies; it must produce a diverse and durable immune memory. Our Antibody Repertoire Sequencing services allow researchers to track the persistence of specific clones over months or years, providing a quantitative metric for vaccine-induced immunity.
In the era of PD-1/PD-L1 inhibitors, understanding the TCR repertoire of Tumor-Infiltrating Lymphocytes (TILs) is critical. MemDX™ can identify "convergent" clones—different sequences that likely target the same tumor antigen—which can serve as potent biomarkers for treatment response.
By identifying expanded autoreactive clones in the peripheral blood or synovial fluid, MemDX™ helps researchers understand the underlying drivers of diseases like Lupus or Rheumatoid Arthritis. This facilitates the development of targeted therapies that neutralize specific clones without compromising the entire immune system.
With over 20 years of experience, Creative Biolabs is more than a service provider; we are your scientific collaborators.
Creative Biolabs offers a modular suite of repertoire analysis services tailored to address diverse research questions:
Optional antibody fragment repertoire analysis services:
Creative Biolabs is committed to excellence in immune repertoire research. With MemDX™ Antibody Repertoire Analysis Services, we provide the clarity and depth you need to advance the next generation of biotherapeutics.
Reference
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
