Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsThe exploration of the immunopeptidome—the vast array of peptides presented by Major Histocompatibility Complex (MHC) molecules—is the cornerstone of modern precision immunotherapy. At Creative Biolabs, we leverage over two decades of biotechnology expertise to offer a comprehensive Peptide Repertoire Analysis platform. By integrating ultra-high-resolution mass spectrometry with advanced bioinformatics, we provide a definitive Peptide Repertoire Solution for researchers targeting cancer neoantigens, autoimmune epitopes, and infectious disease vaccine candidates.
The peptide repertoire, or immunopeptidome, consists of thousands of endogenous peptides bound to MHC (HLA in humans) complexes on the cell surface. These peptides serve as the "molecular window" through which the immune system monitors cellular health. Accurate Peptide Repertoire Profiling is essential for:
Creative Biolabs offers an end-to-end pipeline for the isolation, identification, and validation of peptide–MHC complexes. Our services are designed to overcome the inherent challenges of low abundance and high polymorphism in peptide repertoires.
Utilizing state-of-the-art systems, we perform deep Peptide Repertoire Sequencing. This allows for the unbiased identification of the entire peptidome from tissue samples, cell lines, or biological fluids.
Traditional database searching often fails to identify non-canonical peptides or novel neoantigens. Our Peptide Identification via Spectral Sequencing employs de novo sequencing algorithms and proteogenomic integration to uncover:
We provide specialized protocols for the isolation of MHC-I (HLA-A, B, C) and MHC-II (HLA-DR, DQ, DP) bound peptides. Through immunoaffinity purification using allele-specific antibodies, we ensure the purity and relevance of the extracted peptide repertoire.
Our platform utilizes a multi-dimensional approach to ensure no peptide is missed. The workflow integrates:
We utilize a proprietary library of high-affinity monoclonal antibodies to capture peptide–MHC complexes. Our optimized acid elution protocols minimize salt interference and maximize peptide recovery, which is critical for endogenous peptides bound to MHC.
Before mass spectrometry, peptides are fractionated using liquid chromatography to reduce sample complexity. This increases the dynamic range and allows for the identification of rare, low-copy-number peptides that are often biologically significant.
Raw MS/MS data is processed through our customized suite, which includes:
Fig. 1 Comparison of 96-well- and column-based immunoprecipitation methods.1
The most impactful application of Peptide Repertoire sequencing is the direct identification of neoantigens. While genomic sequencing (WES) predicts potential mutations, only immunopeptidomics proves which mutated peptides are actually presented on the tumor cell surface.
For pandemic preparedness and chronic viral infection research, we map the viral peptides presented by infected cells, facilitating the design of T-cell-inducing vaccines.
We analyze whether biologic drugs (like monoclonal antibodies) are processed and presented as "foreign" peptides by MHC molecules, potentially leading to Anti-Drug Antibodies (ADA).
With over 20 years in the biotech industry, Creative Biolabs is not just a service provider; we are your scientific partner. Our expertise in protein engineering and immunology allows us to interpret Peptide Repertoire Analysis data within the context of your specific therapeutic goals.
Creative Biolabs offers a modular suite of repertoire analysis services tailored to address diverse research questions:
Ready to decode the immunopeptidome? Contact our team of experts to discuss your Peptide Repertoire Analysis project. We provide free initial consultations to help you design the most effective experimental strategy.
Reference
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
