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Quadroma Cell Line Preparation Service

The first trifunctional antibody successfully produced from quadromas is an anti-EPCAM x anti-CD3 bispecific antibody. At first, mouse hybridoma cells whose monoclonal antibodies target one of the desired antigens are produced. Independently, rat hybridoma cells targeting the other antigen are produced. Then, these two cell types are hybridized, yielding hybrid-hybridomas or quadromas, which produce hybrid (trifunctional) antibody as well as pure mouse and pure rat antibody. The trifunctional antibody is extracted chromatographically with protein A.

The disadvantage of quadromas is the produced antibodies are a mixture. Especially if these two hybridoma cells are the same species, up to ten different kinds of antibody can be produced, most of which have mismatched heavy or light chains, or both. Fig. 1 below presents the possible combinations of light- and heavy-chains in antibodies produced by quadroma cell lines.

Fig.1 The illustration of quadroma technology.Fig.1 Antibodies produced by quadroma cell lines.1

Scientists in Creative Biolabs have successfully established the method to produce custom bispecific antibody according to client's requirement. If interested, please contact us.

Reference

  1. Guo, Xiaohan, et al. "Revolutionizing cancer immunotherapy: unleashing the potential of bispecific antibodies for targeted treatment." Frontiers in Immunology 14 (2023): 1291836. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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