Introduction of ABCB1
ABCB1 encoded by ABCB1 gene is a member of the superfamily of ATP-binding cassette (ABC) transporters which transport various molecules across extra- and intracellular membranes. There are seven subfamilies in ABC superfamily named as ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White, respectively. ABCB1 protein belongs to MRP subfamily with a typical structure of transporters. It possesses 12 transmembrane domains forming a channel pore and a nucleotide-binding domain for ATP-dependent translocation of substrates across the cell membrane.
|Basic Information of ABCB1|
|Protein Name||Multidrug resistance protein 1|
|Aliases||CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170|
|Organism||Homo sapiens (Human)|
Function of ABCB1 Membrane Protein
ABCB1 is an ATP-dependent drug efflux pump that is responsible for transport endo- and xenobiotic compounds from cells into the extracellular spaces or out of the body. Hence, it plays an important role in gastrointestinal absorption, distribution, metabolism, excretion, and toxicity of its substrates. ABCB1 in the intestinal epithelium transport xenobiotics (such as toxins or drugs) back into the intestinal lumen. In liver cells, ABCB1 pumps xenobiotics into bile ducts. In the cells of the proximal tubule of the kidney, ABCB1 pumps xenobiotics into urinary filtrate (in the proximal tubule). In the capillary endothelial cells composing the blood-brain barrier and blood-testis barrier, ABCB1 pumps xenobiotics back into the capillaries. Moreover, ABCB1 expressed in the adrenal cortex has been revealed a role in hormone traffic and homeostasis. ABCB1 may also be involved in the transport of cytokines and enveloped viruses in lymphocytes and lipid homeostasis in the periphery and central nervous system. In addition, some studies have shown that ABCB1 is associated with the progression of cancer and the overexpression of ABCB1 is observed in the numerous tissues transformed by cancer.
Fig.1 Structure of the mouse P-glycoprotein based on the 3g5u PDB coordinates.
Application of ABCB1 Membrane Protein in Literature
The study reveals a polymorphism c.3435C>T in ABCB1 is associated with linezolid pharmacokinetics, suggesting a new sight for pharmacogenetic analyses in antimicrobial chemotherapy.
The study shows that the expression levels of ABCB1 and ABCG2 are changed in the rat brain area when rats are chronically exposed to commonly abused drugs, ethanol, and cocaine.
The study shows that the decreased expression of ABCB1 is associated with the accumulation of Aβ which lead to a decreased level of pro-inflammatory cytokines in microglia. This suggests a role of ABCB1 in the pathogenesis of Alzheimer's Disease.
The study reveals that lncRNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) regulates breast cancer paclitaxel resistance through miR-206/ABCB1, which helps to explain the breast cancer chemoresistance.
The study shows that ABCA1 coordinated with ABCB1 promotes arsenic resistance in arsenic-resistant ECV-304 (AsRE) cell line.
ABCB1 Preparation Options
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