Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsIn vivo studies in mouse models, particularly syngeneic or disease-specific models, are non-negotiable for evaluating efficacy, pharmacokinetics (PK), and pharmacodynamics (PD). However, introducing a non-murine antibody (such as a human, humanized, or rabbit antibody) into an immunocompetent mouse triggers a potent Mouse Anti-Drug Antibody (ADA) response. This response neutralizes the therapeutic, rapidly clears it from circulation, and ultimately invalidates the study. To bridge this critical translational gap, Creative Biolabs offers an expert-driven Antibody Murinization (Convert to Mouse Ab) Service. Leveraging over two decades of protein engineering expertise, we intelligently re-engineer your parental antibody by transplanting its binding loops (CDRs) onto a meticulously selected mouse antibody framework. The resulting murinized antibody is recognized as "self" by the mouse immune system, ensuring minimal immunogenicity, an optimized serum half-life, and the acquisition of precise, reliable preclinical data.
Testing a novel human therapeutic antibody directly in a standard mouse model is a flawed approach. The mouse immune system immediately recognizes the human antibody as a foreign protein, leading to a cascade of problems that compromise data integrity. When a human antibody is injected into a mouse, it elicits what is known as a HAMA (Human Anti-Mouse Antibody) response, or more accurately in this context, a MAHA (Mouse Anti-Human Antibody) response.
Antibody Murinization is a sophisticated antibody engineering process that modifies a non-murine antibody (e.g., human, rabbit, rat, or llama) to make it structurally and immunologically similar to a native mouse antibody. This process is, in effect, the reverse of antibody humanization.
The primary goal is to reduce or eliminate the immunogenicity of the antibody when administered in vivo to mice. This is most often achieved through Complementarity-Determining Region (CDR) grafting. The process involves:
The final murinized antibody retains the exact binding specificity of your original antibody but presents a "mouse-like" exterior, thereby evading detection and neutralization by the host immune system.
Simple CDR-grafting is often insufficient. Naïvely placing human CDRs onto a mouse framework can disrupt the delicate canonical structure of the binding loops, leading to a partial or complete loss of affinity.
Creative Biolabs has overcome this challenge with our proprietary antibody murinization platform. This is an integrated, multi-step process that combines sophisticated in silico modeling with rational, structure-guided engineering to guarantee the function of your final murinized antibody.
We begin by sequencing the parent antibody (if necessary). Using its variable domain sequence, our platform scans extensive databases of mouse germline and mature antibody frameworks. We don't just pick the one with the highest sequence identity; we identify the acceptor framework that offers the highest structural homology for the CDR canonical loops.
We generate a high-resolution 3D homology model of the grafted antibody. Our expert team analyzes this model to identify key framework residues (e.g., in the Vernier or "interface" zones) that are critical for supporting the conformation of the parental CDRs. If the chosen mouse framework lacks these crucial residues, we perform rational "back-mutations," re-introducing the original parental residue at that specific position. This step is essential for preserving the original antibody's high affinity and specificity.
The in vivo function of an antibody is dictated by its Fc region. Our Antibody Murinization (Convert to Mouse Ab) Service provides you with complete control over the desired effector functions. We can engineer your antibody onto any mouse isotype, including:
The engineered gene is synthesized, codon-optimized for mammalian expression (HEK293 or CHO), and cloned. We produce a pilot batch of your murinized antibody, purify it to >95%, and perform rigorous quality control. Most importantly, we validate its binding kinetics against the parental antibody.
The primary use of a murinized antibody is to serve as a surrogate for a human therapeutic, enabling critical preclinical studies in immunocompetent mouse models.
When you choose Creative Biolabs to convert to mouse Ab format, you are partnering with a global leader in antibody engineering.
To support your research from discovery to preclinical validation, Creative Biolabs also offers a comprehensive suite of related services:
Don't let ADA responses invalidate your in vivo research. Partner with Creative Biolabs to develop a high-affinity murinized antibody surrogate.
Q: What is the difference between a "chimeric mouse antibody" and a "fully murinized antibody"?
A: A chimeric mouse antibody is a simpler construct created by fusing the entire parental variable domains (VH and VL) to a mouse constant region (Fc). While this reduces immunogenicity, the entire non-mouse variable region can still be immunogenic. A fully murinized antibody, created via CDR-grafting, is much more "mouse-like." It only contains the 6 small CDR loops from the parent, while the entire framework (FR) and constant region are of mouse origin. Murinization is the gold-standard method for minimizing immunogenicity for in vivo studies.
Q: I only have the hybridoma cell line, not the sequence. Can you still perform the Antibody Murinization Service?
A: Yes. This is a very common starting point. We would first perform our De Novo Antibody Sequencing Service using your hybridoma cell line or purified antibody. Our high-fidelity sequencing platform will identify the precise VH and VL sequences, which we will then use as the starting material for the Antibody Murinization (Convert to Mouse Ab) Service.
Q3: Which mouse isotype should I choose: mIgG1 or mIgG2a?
A: This is one of the most important decisions and depends entirely on your therapeutic's intended mechanism of action (MOA).
Our scientific team will consult with you to select the optimal isotype for your project.
Q: How do you guarantee the affinity of the murinized antibody?
A: We stand by our antibody murinization platform. The affinity of an antibody is determined by the CDR loops and the framework residues that support their structure. Our advanced in silico modeling and structure-guided back-mutations are designed to preserve this precise 3D architecture. We provide a side-by-side validation report that directly compares the binding kinetics of your original antibody and our final murinized antibody, ensuring they are comparable.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
