Cell-based Membrane Protein Expression and Purification

Membrane proteins partially interact with or completely embed into the cell membrane. They participate in a wide variety of biological processes including ligand-receptor binding, signal transduction, molecule transportation, intercellular recognition and cell adhesion, etc. Of particular interest, they constitute around 50-60% of current drug targets, mainly GPCRs, ion channels, transporters.

Cell-based protein expression (Creative Biolabs)

Cell-based Membrane Protein Expression

Based on innovative technologies and extensive experience, we developed multiple cell-based expression platforms aiming to overcome the major difficulties in membrane protein production. The expression platforms are highly dynamic for the multiple options of host organisms to fulfill diverse custom demands.

E. coli expression system (Creative Biolabs)

Yeast expression system (Creative Biolabs)

Baculovirus-Insect Cells expression system (Creative Biolabs)

Mammalian Cells expression system (Creative Biolabs)

Photosynthetic Bacteria expression system (Creative Biolabs)

Lactococcus Lactis expression system (Creative Biolabs)

We can perform expression, purification, stabilization, and characterization using a broach range of approaches. Many thanks to the efforts of our outstanding R&D team, a series of novel reagents have been utilized to provide a non-denaturing environment for generation of full-length membrane proteins with native conformation and sufficient bio-activity. Our customized membrane protein products can perfectly meet the requirement for antibody development, structural determination, functional and mechanism studies, and various other applications.

Expression System	Lead Time	Yield	Growth Medium	Protein Folding	Post-Translational Modifications
E.coli cells	Rapid	High	Simple, inexpensive	Refolding may be required	No
Photosynthetic bacteria cells	Rapid	High	Simple, inexpensive	Refolding may be required	No
Yeast cells	Moderately rapid	High	Simple, inexpensive	Refolding may be required	Yes
Baculovirus-insect cells	Relatively slow	Moderate	Complex, expensive	Proper folding	Yes (not all)
Mammalian cells	Relatively slow	Moderate	Complex, expensive	Proper folding	Yes

Membrane Protein Purification

Our scientists in Creative Biolabs have devoted to various optimizations and exploration to the process of membrane protein preparation, from solubilization, purification to characterization. With years of experience, we have gained remarkable success in obtaining a variety of difficult membrane proteins such as G-protein coupled receptors (GPCRs), ion channels, membrane transport proteins, etc.

One of the hallmarks of membrane proteins is their hydrophobic transmembrane domains. Due to their hydrophobic feature, membrane proteins tend to aggregate, especially when they are misfolded. In order to keep membrane proteins homogeneously dispersed in aqueous solutions with their native functions intact, it is almost inevitable to apply micelles, cell membrane-like lipid bilayer systems (bicelles, liposomes, or nanodiscs) or polymers to solubilize and stabilize them.

Membrane Protein Purification Form (Kermani, 2021)

Creative Biolabs now offers professional cell-based protein expression and purification services to our worldwide clients. Our seasoned scientists are always ready to off first-class assistance to best facilitate our clients' research goals. As an alternative, Creative Biolabs also provides Membrane Protein Products and Cell-free Membrane Protein Expression service. Please feel free to inquiry us for further discussions.

Reference

Kermani, A.A. A guide to membrane protein X-ray crystallography. FEBS J. 2021, 288(20):5788-5804.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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