Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate

So far, cell-free membrane protein expression (CFMPE) systems have been used for decades as a research tool and have introduced many attractive advantages to membrane protein expression technology. However, a critical barrier to the adoption of CFMPE systems as alternatives to cell-based approaches has been the problem of relatively low protein yields. To address this problem, we have taken advantage of plant cell biology by developing an advanced eukaryotic cell-free expression system that provides unprecedented yields of up to 3 mg/mL of protein in batch mode. This method can help researchers to overcome the limitations of living host systems and to obtain "difficult to produce" membrane proteins in a matter of hours instead of weeks.

Kit Description

Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate Kit utilizes tobacco cell lysates containing all factors required for in vitro transcription and translation reactions. Using a simple protocol, both the RNA transcription and translation take place in a single tube. Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate Kit comes with two expression vectors, Vector1 and Vector2. Vector1 is used for expression in cytosolic fraction. Targeting the microsomes with Vector2 offers the opportunity to express protein with post-translational modifications like disulfide bonds.

Components List and Storage

Expression System	Components	Storage
In Vitro Plant Cell Expression System	Master Mix	-80°C
	Vector1	-20°C to -80°C
	Vector2	-20°C to -80°C
	Tubes	Room Temperature

Features

High-yield: Up to 3 mg/mL expressed protein
Easy to use: One-step reaction protocol
Fast: High yield in just 48 hours
Flexible: Express "difficult to produce" membrane proteins, cytotoxic proteins, etc.
Convenience: Suitable for various membrane protein expressions

Applications

This kit can be used for large-scale expression of target membrane proteins. Thus, there are listed many other additional applications, including protein optimization, target protein, mutant screening protein-protein interactions, expression analysis, protein localization analysis, metabolomics, protein structure analysis, herbicide screening, post-translation modification assays.

Fig.1. Applications of Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate. (Creative Biolabs)

At the current state of the art, lysates from the plant have been successfully used for the production of proteins. Creative Biolabs is a world-leading service provider of cell-free membrane protein expression system. We now provide integrated service of Magic™ cell-free membrane protein production in plant cell lysate for clients around the world. To get more information, please feel free to contact us.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-7	Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate	3 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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