Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights
As pioneers in antibody development and engineering, Creative Biolabs understands that a comprehensive understanding of an antibody's biophysical and functional properties is the cornerstone of therapeutic and diagnostic success. For over 20 years, our dedicated team of PhD-level experts has provided best-in-class antibody property analysis services, empowering researchers worldwide to de-risk their candidates, accelerate development timelines, and meet stringent regulatory standards. Our state-of-the-art platforms and customized assay designs provide a complete, 360-degree view of your antibody, from initial discovery and screening to lead optimization and pre-clinical evaluation. We translate complex molecular interactions into actionable data, giving you the confidence to advance the most promising candidates.
Antibody affinity (represented by the equilibrium dissociation constant, Kᴅ) is arguably the most critical attribute for any antibody candidate. It dictates binding strength, specificity, and ultimately, biological efficacy. A precise antibody affinity measurement is non-negotiable for selecting leads with optimal potency and target engagement.
Creative Biolabs offers a suite of cutting-edge platforms to measure a full spectrum of binding kinetics (ka/kon and ke/koff) and affinity (Kᴅ), from picomolar to millimolar ranges.
SPR is the industry's gold-standard for real-time, label-free kinetic analysis. By immobilizing one binding partner and flowing the other over a sensor chip, we can precisely measure the association and dissociation rates, providing high-resolution data on binding kinetics.
BLI is a powerful, high-throughput alternative to SPR. This fluidics-free technology measures changes in the interference pattern of light reflected from a biosensor tip, providing rapid and reliable kinetic data.
Fig. 1 Illustration of Bio-Layer Interferometry.1
ITC stands unique as the only technology that directly measures the heat released or absorbed during a binding event. This allows for a direct, in-solution measurement of affinity without the need for immobilization or labeling, providing a complete thermodynamic profile.
MST is a cutting-edge, solution-based method that measures molecular motion along a microscopic temperature gradient. A change in hydration shell, charge, or size upon binding directly correlates to affinity.
Table 1. Technology Comparison for Antibody Affinity Measurement
| Feature | Surface Plasmon Resonance (SPR) | Bio-Layer Interferometry (BLI) | Isothermal Titration Calorimetry (ITC) | MicroScale Thermophoresis (MST) |
| Principle | Change in refractive index | Change in optical layer thickness | Heat change upon binding | Change in molecular movement |
| Labeling | Label-free | Label-free | Label-free | Labeling or label-free |
| Immobilization | Required | Required | Not Required (in-solution) | Not Required (in-solution) |
| Throughput | Low to Medium | High | Low | Medium to High |
| Kinetics (kon/koff) | Yes (Gold Standard) | Yes | No (Thermodynamics Only) | Yes (Kᴅ primarily) |
| Sample Type | Purified | Crude & Purified | Purified | Crude & Purified |
| Key Advantage | High-resolution kinetic data | High throughput, crude samples | Complete thermodynamic profile | Low sample usage, in-solution |
Beyond affinity, a successful antibody candidate must possess a robust profile of biophysical, structural, and functional properties. Creative Biolabs provides a comprehensive suite of services to complete your characterization package.
We believe in a collaborative approach, tailoring every project to your specific goals.
Navigate to our specialized services to learn more about how we can support every stage of your antibody program:
Other optional characterization Services:
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
