Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsWith the continuous development and deepening of research of antibody technology, the role of antibodies in the treatment of diseases, disease diagnosis, disease mechanism research, and other fields has become more and more significant. Monoclonal human antibodies have significant advantages in diseases, which can eliminate the adverse reaction of humans to heterologous antibodies and avoid the complexity of recombination of heterologous antibodies. Monoclonal antibodies have demonstrated excellent therapeutic effects in the treatment of cancer, viral myocarditis, cytomegalovirus infection, human immunodeficiency virus infection, and many other diseases. Based on our rich field experience and advanced antibody preparation platform, Creative Biolabs provides comprehensive antibody discovery services to support monoclonal human antibody production.
The first monoclonal antibodies prepared were mainly based on murine-derived monoclonal antibodies. Mouse-derived monoclonal antibodies are secreted by hybridoma cells formed by the fusion of B lymphocytes from immunized mice with mouse myeloma cells. The human immune system recognizes this antibody and produces a severe human anti-mouse antibody response. This greatly limits the application of monoclonal antibodies. Therefore, scientists began to explore humanized monoclonal antibodies. Among chimeric monoclonal antibodies, humanized monoclonal antibodies, and fully humanized monoclonal antibodies, the latter can eliminate adverse human reactions to heterologous antibodies. This has greatly expanded the diagnostic and therapeutic role of monoclonal antibodies in human diseases.
Phage display technology is an effective method for the preparation of monoclonal human antibodies. It involves reverse transcription of the genes of heavy and light chain variable regions in the structure of human antibodies, transfection of human antibody genes into phage vectors, and expression in the form of fusion proteins. By this technique, phages containing antibody genes and capable of successfully expressing antibodies can be obtained. Moreover, the preparation of antibodies by phage display technology takes less time and does not require animal culture. However, phage display library construction and screening are the key and difficult points of this technology. The collection of all phages displaying different exogenous genes is a phage display library. At present, the commonly used methods to construct libraries with nucleic acid fragments of different sequences are synthetic method, cDNA, DNase I randomly hydrolyzed DNA, and so on. The screening of phage display libraries is mainly divided into in vivo and in vitro screening.
Immune antibody libraries are one of the main types of phage antibody libraries. It is constructed using spleens or other organs of animals immunized with specific antigens. As a result of the selective pressure of immunization, the abundance of antibodies in the immune antibody pool that are specific for the corresponding immunogen is considerably higher than that of other non-specific antibodies. Some of these specific antibodies have undergone the affinity maturation process of the immune system, and thus high affinity, high specificity antibodies can be screened from this immune-antibody library. An immune-antibody library is an excellent tool for studying the humoral immunity of an organism to a certain antigen, such as autoimmune diseases and viral infections. An immune-antibody library can be enriched for antigen-specific antibodies, and a high percentage of the library encodes functional antibodies that recognize a specific immunogen, meaning that functional antibodies and their genes can be screened for immunogens from a library with a small library capacity.
Creative Biolabs has a wealth of knowledge and experience in monoclonal human antibody discovery. We would be happy to share with you our knowledge and experience.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
