Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights| Protein Name | Cholinergic receptor muscarinic 3 |
| Gene Name | CHRM3 |
| Uniprot | P20309 (Human); Q9ERZ3 (Mouse) |
| Synonym | HM3; PBS; EGBRS; muscarinic acetylcholine receptor M3; acetylcholine receptor, muscarinic 3; m3 muscarinic receptor |
| Background | CHRM3, encoded by CHRM3 gene, is a member of G protein-coupled receptors family which has seven distinctive transmembrane domains. It is one of five subtypes of muscarinic receptors (CHRM1-5), selectively expressed in various human tissues, including brain, salivary gland, and 12 other tissues. Researchers have found that muscarinic receptors are the potential therapeutic targets for a variety of pathological conditions, especially in neurodegenerative disorders. |
Creative Biolabs offers comprehensive range of membrane protein products empowers your unique research needs.
| CAT# | Product Name | Expression System | Protein Length | Solubilizing Agents |
| S01YF-1023-KX334 | NativeExtract™ Human CHRM3 Membrane Protein (Full length, Super Nanodisc) | HEK293 cells | Full length | Native Nanodisc |
| MP0219X | MemDX™ Membrane Protein Human CHRM3 (Cholinergic receptor muscarinic 3) for Antibody Discovery | Wheat germ cell-free | Full length | Liposome |
| MPX4617K | MemDX™ Membrane Protein Human CHRM3 Expressed in E.coli with 6xHis and B2M tag at the N-terminus, Partial (253-492aa) | E.coli | Partial | N/A |
| MPX4329K | MemDX™ Membrane Protein Human CHRM3 Expressed in Yeast with 6xHis tag at the N-terminus, Partial (253-492aa) | Yeast | Partial | N/A |
| Please for more products or customization | ||||
C***y: I've worked with Creative Biolabs on multiple projects, and each time, they've delivered excellent results.
09/Oct/2023
R***y: The team is highly responsive and always ready to provide guidance and support. I highly recommend their services for anyone seeking cutting-edge solutions in the biotech field.
24/Aug/2024
We provide tailored solutions to meet your specific requirements. Please for more services.
Lentivirus-Mediated Overexpression
Creative Biolabs offers lentivirus-mediated overexpression development services. Beyond their application in stable gene integration for long-term expression, lentiviral vector systems also furnish a robust and widely utilized platform for inducing the ectopic overexpression of specific molecular targets, such as the CHRM3, within a multitude of cellular contexts. This methodological strategy proves instrumental, as it facilitates the detailed interrogation of CHRM3's intrinsic physiological functions and allows for an initial, yet critical, assessment of its putative therapeutic effects or liabilities. Indeed, such lentivirally-mediated overexpression of CHRM3 has been effectively employed across a diverse spectrum of established in vitro cell lines. These investigations have frequently been directed towards meticulously dissecting the nuanced involvement of CHRM3 in fundamental cellular processes.
Plasmid Construction and Transfection
Creative Biolabs offers plasmid construction and transfection services. A foundational experimental strategy for interrogating gene function involves the generation of plasmid constructs specifically engineered to harbor the coding sequence of a gene of interest. These plasmid vectors are subsequently introduced into appropriate recipient cells, typically via established transfection methodologies, with the explicit aim of inducing CHRM3 overexpression. This controlled induction of protein overabundance then provides a valuable experimental system.
Creative Biolabs offers comprehensive and innovative services to drive the development of CHRM3 cell therapy. Please for more services.
Proliferation Assays
Creative Biolabs offers proliferation assays services. To quantitatively evaluate the influence of Cholinergic Receptor Muscarinic 3 (CHRM3) expression levels on cellular growth dynamics, a frequently adopted methodological approach involves the meticulous monitoring of cell proliferation over time. Specifically, the Cell Counting Kit-8 (CCK-8) assay, a sensitive colorimetric method reliant on the bioreduction of WST-8 by cellular dehydrogenases, is often employed for this purpose. This technique facilitates the precise determination of viable cell numbers, and consequently cellular proliferative rates, at designated intervals across a defined temporal spectrum.
Migration and Invasion Assays
Creative Biolabs offers migration and invasion assays services. The assessment of cellular migratory potential is frequently undertaken utilizing in vitro wound healing assays. These established methodologies furnish a quantifiable measure of collective cell movement, providing critical data when examining cellular responses to perturbations, such as the experimental modulation of Cholinergic Receptor Muscarinic 3 (CHRM3) expression levels. Such analyses primarily illuminate parameters associated with two-dimensional cell migration. Nevertheless, the insights derived from these experiments are often considered highly pertinent when investigating the broader suite of cellular attributes.
Immunofluorescence Staining
Creative Biolabs offers immunofluorescence staining services. The precise determination of protein distribution within both individual cells and complex tissue architectures is fundamental to understanding biological function. Methodologies such as immunofluorescence microscopy serve as powerful tools for achieving this, enabling the high-resolution spatial localization of specific molecular targets, for instance, the CHRM3.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
