In vivo Electroporation-Mediated DNA Immunization Service

Electroporation also refers to electropermeabilization or EP for short. Through the appropriate electrical stimulation of target cells, this technology can induce the transient transfection and increase cellular uptake of interested DNA. It has been widely used for in vitro transfection (e.g. bacteria, yeast, and plant protoplasts transformation) but can also be a qualified in vivo DNA delivery method. The in vivo EP starts with the administration of interested DNA by intramuscular or intradermal injection and then immediately applies electric pulses to the injection site by using appropriate device.

Intratumoral injection of plasmid followed by in vivo electroporation. The interested DNA is administrated by intramuscular or intradermal injection firstly, and then immediately applies electric pulses to the injection site by using appropriate device.

For genetic immunization, in vivo electroporation is a reliable approach which can strongly improve the delivery and duration of interested DNA into the cell. During the directly DNA injection of host animals, a lower immune response may be suffered due to the insufficient DNA transfection efficiency. Electroporation, in this case, can be a potential solution to overcome the limitation. This technique can transiently enhance the permeability of the cell membrane to increase the transfection efficiency. During the delivery, this approach can also lead to mild inflammation which contributes to the influx of antigen-presenting cells (APCs) and the exposure of the expressed foreign protein. On account of fewer resident APCs exist in the muscle tissue (compared with the skin), the induced inflammation via in vivo electroporation likely achieves much higher transfection efficiency and more robust immune response in comparison to the direct intramuscular/intradermal injection, especially for the immunization of large animals. In fact, in vivo electroporation can increase the expression of exogenous gene encoding protein 10 to 100 folds more than a direct injection of naked DNA.

Electroporation jolts embryos with electricity to open holes that allow the interested gene (upper right) to enter and edit the DNA.

Features of in vivo Electroporation

Available for a variety of host species without serious safety concerns
Enhance the transfection efficiency
Produce inflammatory cytokines and recruit APCs
Induce more robust immune response than simple intramuscular injection
Induce Th1-type immune response

Antibody Discovery via in vivo Electroporation

As one of high-efficiency DNA immunization approaches, in vivo electropermeabilization has been employed as an alternative technique for a series of antibody development services at Creative Biolabs. Similar with other DNA immunization method, electroporation can sufficiently generate antibodies recognize in vivo expressed antigens and resulting in longer lasting immune response. According to the advanced antibody production platform and decades of antibody research experience, scientists of Creative Biolabs can combine our most comprehensive technologies with this technique to generate high-quality antibodies for our global clients.

Comprehensive Antibody Production Platforms of Creative Biolabs

In addition to the in vivo electroporation method, DNA immunization services via other techniques are also available at Creative Biolabs, which including but not limited to:

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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