Gene-Inactivated Mice Introduction

Gene-inactivated mouse hybridoma technology is an important approach to developing antibodies using hybridoma technology with gene-deficient mice. This approach offers unique advantages in antibody production compared to hybridoma techniques in wild-type mice. In practical research and production, appropriate hybridoma methods can be chosen based on specific research needs.

The Necessity of Hybridoma Generation in Gene-Inactivated Mice

Although the hybridoma technique developed by Kohler and Milstein is well-established and widely used in biology, it has some drawbacks.

In general, the greater the phylogenetic distance between antigen and receptor, the weaker the immune response. Highly conserved mammalian proteins usually elicit weak immune responses. Lymphocytes carrying the autoantigen receptor are not available (by active clearance or functional inactivation), murine monoclonal antibodies against the mouse protein (the autoantigen) cannot be expected to be produced. Similarly, the immune system fails to recognize epitopes in proteins from other species that show a high degree of homology to epitopes in the corresponding proteins of the immune species.

Therefore, scientists have developed gene-inactivated mouse hybridoma technology to address the problem of sequence similarity between species in antibody production.

The Advantages of Genetically-Inactivated Mice

Direct immunization of gene-deficient mice by deletion of the gene results in a strong immune response and greatly enhanced antibody concentrations in the serum of the mice compared with the production of antibodies by wild-type mouse hybridoma technology. Therefore, the hybridoma technique for antibody production in genetically inactivated mice tends to yield more antibodies. In general, wild-type mouse hybridoma techniques fail to produce consistent antibodies against autoantigens because clonal selection eliminates functional lymphocytes carrying autoantigen receptors. In addition, induced and/or selected antibodies target epitopes that are not conserved between the two species. In contrast, hybridoma technology in genetically inactivated mice covers a wide range of epitopes located in different domains of the molecule and is conserved across a variety of species. Immunization of any type of genetically inactivated mice with the respective knockout proteins could provide a general means to obtain unique monoclonal antibodies against structurally and functionally conserved domains in other protein families.

Antibody Production from Hybridoma Based on Genetically Inactivated Mice

The process of antibody production in hybridomas of gene-inactivated mice is similar to that of general mouse hybridomas. The deletion gene is dissolved in complete Freund's adjuvant, and then gene-inactivated mice are immunized. Clear intervals are determined according to the specific experimental protocol, and then genetically inactivated mice are reimmunized using incomplete Freund's adjuvant. When the antibody titer in the serum meets the requirements, the serum is collected, and the antibodies are detected. Large-scale antibody production is decided based on demand.

Gene-inactivated mouse hybridoma technology has unique advantages and characteristics in the field of antibody production. Creative Biolabs is willing to discuss the experience and difficulties in the field of gene-inactivated mouse hybridomas, to promote growth and success.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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