With years of research and development experience in the field of immunology, Creative Biolabs has owned a group of scientists who have deep understanding of the adaptive immune system. Through our advanced one-stop BCR repertoires analysis service, our seasoned scientists can provide the best service of BCR repertoire profiling in swine. We are proud to share our expertise and experience with our global clients to facilitate their related immune repertoire researches.
The Properties of Antibody Repertoire in Swine
Studies have shown that newborn swine utilize primarily four VH genes and two DH segments to generate their preimmune natural antibody repertoire. In the fetus, VDJ rearrangements are ﬁrst seen in yolk sac at 20 days of gestation. However, signal joint circles are difﬁcult to recover currently, probably because of the slow rate of B cell lymphogenesis at this early time and the rapid rate of their degradation. Since swine have only a single JH, 30 combinatorial diversity is limited to essentially eight combinations. VDJ rearrangement was first observed on day 30 in fetal swine and all major isotypes of immunoglobulins are transcribed in utero. Swine has been used to better understand B-cell lymphogenesis in biomedical research, especially where antibody responses are involved. The piglet model has provided useful information on the B cell system for studies on antibody repertoire development. The process is too complicated and the results are always inconsistent with each other.
Fig.1 The critical window of immunological development. The factor affecting events in the “window” are indicated. There are a number of cases of superimposing events; e.g., Passive immunity (red) superimposed on innate immunity (green) produces yellow. The “lined section” prepartum applies only to species, e.g., mice and humans, in which passive immunity also takes place in utero. In Ungulates like swine, there is no transfer of passive antibodies in utero (John & Nancy 2012).
Swine BCR Repertoire Profiling in Creative Biolabs
High-throughput NGS has been widely used for the study of antibody repertoires. The diversity of the sequences obtained from these studies is immense, and thus efficient bioinformatics techniques to analyze and interpret the data are also required. Creative Biolabs has established the high-throughput Magic™ platform for antibody repertoires analysis in swine. Our scientists have also designed advanced algorithm for analyzing Ig gene sequences in swine obtained by sequencing. With high throughput sequencing and superior bioinformatics analysis tools, our Magic™ platform is powerful in analyzing a large number of DNA sequences present in the expressed antibody repertoire.
Key Advantages of Our Swine BCR Repertoire Profiling Service
Equipped with world-leading technology platforms and professional scientific staffs, Creative Biolabs has rich experience in BCR repertoire profiling in swine. We are pleased to share our cutting-edge technology and extensive expertise in BCR repertoire profiling in swine to facilitate our clients’ research and project development.
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