Creative Biolabs provides reliable, large-scale manufacturing of targeted delivery systems under strict quality control, ensuring consistency for your programs.
The future of medicine depends on our ability to deliver treatments with pinpoint accuracy. While conventional delivery systems struggle with biological barriers, off-target effects, and payload instability, extracellular vesicles—especially exosomes—offer
a revolutionary breakthrough. At Creative Biolabs, we draw on over two decades of expertise to transform this potential into practice, delivering a comprehensive platform for Exosome-based Delivery System
Development.
The Power of a Natural Nanocarrier
Fig. 1 Schematic diagram of the structure of
exosomes.1
Exosomes are lipid-bilayer nanoparticles secreted by cells, playing a vital role in intercellular signaling. Typically 30–150 nm in size, they transport diverse molecular cargo, including proteins, lipids, and nucleic acids (e.g., mRNA, miRNA, DNA). Due
to their natural biocompatibility and transport efficiency, exosomes are uniquely suited to serve as nanocarriers in cutting-edge drug delivery platforms.
Unlike synthetic delivery vehicles, exosomes possess inherent biological advantages that make them a superior choice for drug delivery:
Innate Biocompatibility: Derived from the body's own cells, exosomes exhibit low immunogenicity, minimizing the risk of adverse immune reactions.
Effective Barrier Penetration: Their natural origin and small size enable them to navigate complex biological barriers, including the blood-brain barrier (BBB), making them ideal for challenging therapeutic targets.
Intrinsic Stability: The exosomal membrane protects sensitive payloads from enzymatic degradation, ensuring drug stability in circulation.
Targeting Potential: Their surface proteins can be engineered to achieve highly specific and active targeting of disease-relevant cells and tissues.
One-Stop Solution for Exosome Development
Contact an Expert
Our services are built on a robust, modular platform that combines module delivery systems with targeted modules. This approach allows for unparalleled customization and flexibility, ensuring your exosome delivery system is optimized for your specific
therapeutic payload and target.
Exosome Isolation & Purification
Our service ensures the isolation of highly pure and functional exosomes from a diverse range of sources. This service is ideal for basic research, biomarker discovery, and preparing starting material for downstream applications.
Sample Sources
We handle challenging and diverse samples, including cell culture supernatants, serum, plasma, cerebrospinal fluid, ascites, pleural effusion, urine, and various tissue samples.
Key Methods
We employ gold-standard techniques such as differential ultracentrifugation, size exclusion chromatography (SEC), and precipitation-based methods to achieve high purity and yield.
Quality Control
Each isolated batch undergoes rigorous quality control using a suite of analytical tools to ensure consistency and biological integrity.
Deliverables
A high-purity, functional exosome suspension accompanied by a detailed QC report.
Exosome Characterization & Analytics
We provide comprehensive analysis to validate the identity, purity, and quality of your exosome preparations. This service is critical for quality control, publication-ready data, and ensuring batch-to-batch consistency.
Morphology
Transmission electron microscopy (TEM) for definitive visual confirmation of morphology and to assess sample purity.
Size & Concentration
Nanoparticle tracking analysis (NTA) for quantitative measurement of particle size distribution and concentration.
Marker Profiling
Western blotting for specific exosome markers (e.g., CD9, CD63, TSG101) and flow cytometry for single-particle surface marker validation.
Nucleic Acid Quantification
PCR-based analysis to quantify specific nucleic acid cargo within exosomes, including miRNA, mRNA, and DNA.
Exosome Cargo Loading
We provide specialized solutions for the efficient and protective encapsulation of various therapeutic payloads into exosomes. This service is essential for developing novel drug delivery systems for gene therapy, oncology, and regenerative medicine.
Payloads
We expertly load a wide range of molecules, including nucleic acids (mRNA, siRNA, plasmids), small molecules, extracellular proteins, and others.
Loading Methods
We utilize proprietary and established techniques, such as electroporation, sonication, and chemical-based methods, to maximize encapsulation efficiency.
Payload Integrity
Our optimized protocols ensure that the therapeutic cargo remains stable and functional post-loading, protecting it from degradation.
Customizable Solutions
We work collaboratively to select the most suitable loading method based on your specific payload and downstream application.
Engineered Exosome Development
Our platform allows for the customization of exosome surfaces to achieve enhanced targeting and therapeutic efficacy. This service is ideal for applications requiring enhanced targeting to specific organs, cells, or disease sites, such as crossing the
blood-brain barrier.
Genetic Engineering
We engineer donor cells to overexpress specific proteins or targeting ligands that are subsequently incorporated onto the exosome surface during biogenesis.
Chemical Modification
Our chemical conjugation services allow for the direct attachment of targeting molecules to the exosome membrane using precise linkers.
Functional Enhancement
Engineered exosomes exhibit enhanced targeting specificity, improved cellular uptake, and the ability to cross biological barriers more effectively.
Exosome Omics & Profiling
Unlock the full potential of exosome research by characterizing their molecular cargo with our advanced omics and sequencing platforms. Ideal for biomarker discovery, disease mechanism studies, and companion diagnostics development.
Starting Materials
For optimal data quality and reliable downstream analysis, we require highly purified and well-characterized exosome or other extracellular vesicle preparations.
Sequencing
We offer a suite of sequencing services to profile exosomal nucleic acids, including miRNA, lncRNA, circRNA, mRNA, and whole transcriptome sequencing.
Omics
Our multi-omics solutions—proteomics, lipidomics, and metabolomics—provide a comprehensive understanding of exosomal content.
Bioinformatics Analysis
We provide in-depth bioinformatics and pathway analysis services to help you interpret complex datasets and identify novel biomarkers or therapeutic targets.
Exosomal Function Testing
We offer a suite of robust assays to evaluate the biological impact of your exosome preparations, from in vitro cellular interactions to in vivo therapeutic outcomes.
In Vitro Assays
We provide a full suite of assays to assess key cellular functions, including proliferation, apoptosis, cell cycle, migration, and invasion.
In Vivo Studies
We offer comprehensive in vivo studies to evaluate biodistribution, pharmacokinetics, and therapeutic efficacy.
Safety & Toxicity
We perform biocompatibility and toxicity assays to evaluate the safety profile of your exosome therapy.
Deliverables
A comprehensive functional data and assay report, including images, graphs, and statistical analysis.
Creative Biolabs' exosome-based delivery systems are transforming therapeutic research across multiple disciplines, providing a powerful tool to address complex biological challenges.
Gene Therapy: Exosomes serve as a natural vehicle for the precise delivery of nucleic acid therapeutics, enabling targeted gene silencing and expression for the treatment of genetic disorders
and cancers.
Tissue Regeneration: By carrying proteins and growth factors, exosomes can stimulate cellular proliferation and differentiation, promoting the repair and regeneration of damaged tissues.
Drug Delivery: Exosomes are ideal for delivering a wide array of payloads, from small molecules to complex biologics, to specific cells or organs, enhancing efficacy and reducing systemic toxicity.
Oncology: Enabling targeted delivery of therapeutics to tumor cells, reducing systemic toxicity, and improving treatment outcomes.
Vaccine Development: Exosome-based vaccines can present antigens in a native, highly immunogenic form, eliciting a robust and targeted immune response.
Disease Diagnosis: The molecular cargo within exosomes can serve as a biomarker for various diseases, offering a non-invasive approach to early detection and diagnosis.
Why Choose Creative Biolabs for Your Exosome Research?
Request a Quote
Unrivaled Expertise
With over two decades of experience in biotechnology, we have the scientific knowledge to navigate the complexities of exosome-based therapeutics.
Tailored, Collaborative Approach
We are not a one-size-fits-all vendor. Our services are fully customizable, and we work as a true partner to design and execute a project plan that meets your
specific research goals.
Proprietary Technology
Our advanced, in-house platforms for exosome engineering and high-throughput screening give you a competitive advantage in a rapidly evolving field.
Rigorous Quality Control
Every step of our process is underpinned by stringent QC protocols, ensuring the reliability, consistency, and reproducibility of your results.
Empower your research with a platform that is smarter, more precise, and more effective. Creative Biolabs is ready to help you unlock the full potential of exosome-based therapeutics. Our team is ready to discuss your
specific needs and develop a customized plan that aligns with your research goals. Please reach out to our specialists to learn how our exosome solutions can help you achieve your next breakthrough.
Complementing our comprehensive service offerings, Creative Biolabs provides a portfolio of high-quality products designed to streamline your research and development workflows. Our products are developed with the same
scientific rigor and quality standards as our services, ensuring reliable performance and consistent results for your in-house experiments.
Product Name
Description
Inquiry
Exosome Standards
A range of characterized exosome standards (e.g., derived from milk, plasma, HEK293T cells, MSCs, and NK cells) for method validation, quantification, and quality control.
Inquiry
Exosome Labeling Dyes
High-purity fluorescent dyes and labeling reagents designed for robust and stable in vitro and in vivo tracking of exosomes.
Inquiry
Exosome Preservation Solution
An optimized solution for long-term storage of isolated exosomes, preserving their morphology, biological activity, and cargo integrity.
Inquiry
Lysis Buffer
A specialized buffer formulated for the efficient and complete lysis of exosomes to extract proteins, lipids, and nucleic acids for downstream analysis.
User-friendly kits for rapid and efficient isolation of exosomes from various biological fluids and cell culture media
Inquiry
FAQs
What is the typical turnaround time for a project?
Project timelines vary based on complexity, but our streamlined workflow ensures efficient delivery.
What types of payloads can be loaded into exosomes?
Our platform is versatile and can efficiently encapsulate a vast range of payloads, including small molecule drugs, nucleic acids (siRNA, mRNA, DNA plasmids), proteins, and peptides.
How do you ensure the quality of the exosomes?
We employ a multi-step quality control process, including Nanoparticle Tracking Analysis (NTA) to measure size and concentration, Transmission Electron Microscopy (TEM) for morphological analysis, and Western Blotting for the detection of exosome-specific
markers.
Can you provide animal studies for in vivo validation?
Yes, we offer comprehensive in vivo validation services. We can design and execute animal studies to evaluate the biodistribution, targeting efficiency, and therapeutic efficacy of your engineered exosome system.
Can samples be stored at low temperature before extracting exosomes?
Yes. For optimal preservation of exosomes, samples such as plasma, serum, or cell culture supernatant should be stored at −80°C. Repeated freeze-thaw cycles should be avoided as they can compromise exosome integrity and yield.
Will dead cells during the cell culture process affect the extraction of exosomes?
Yes, the dead or dying cells release other types of extracellular vesicles and cellular debris that can contaminate the exosome sample, reduce purity, and potentially alter experimental results.
Reference
Jan, Arif Tasleem, et al. "Expedition into exosome biology: a perspective of progress from discovery to therapeutic development." Cancers 13.5 (2021): 1157. https://doi.org/10.3390/cancers13051157. Distributed under an Open Access license CC BY 4.0,
without modification.
Our services are For Research Use Only. We do not provide services to individuals.
Fig. 1 Schematic diagram of the structure of
exosomes.1 
