Liposome based Delivery System Development Service
In the pursuit of groundbreaking therapies, the journey from a promising molecule to a viable treatment often stalls at a single, formidable obstacle: targeted delivery. Protecting fragile nucleic acids and guiding therapeutics to their precise site of action are challenges that can make or break a project. At Creative Biolabs, we see this not as your problem, but as our passion. We partner with researchers by offering a fully integrated suite of customized liposome services, providing the expert support and advanced formulations necessary to transform your vision into reality. Together, we can unlock the full potential of your therapeutic candidates.
What Are Liposomes?
Liposomes are microscopic, spherical vesicles formed by one or more lipid bilayers, making them naturally biocompatible. Their core structural feature—an internal water-based core surrounded by a fatty lipid membrane—is the key to their versatility. This dual nature allows them to encapsulate a wide array of therapeutic substances. Hydrophilic (water-loving) drugs can be contained within the aqueous core, while hydrophobic (fat-loving) molecules can be incorporated into the lipid membrane itself. As a result, liposomes serve as highly adaptable carriers, capable of transporting everything from simple small molecules to fragile genetic payloads like mRNA.
The rational design of a successful liposomal delivery system is contingent upon a thorough understanding and precise manipulation of its core physicochemical properties. These parameters are systematically optimized to achieve the desired therapeutic profile for a given application.
Fig. 1 The structure of liposomes.1
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Composition: Liposomes are primarily composed of naturally occurring or synthetic phospholipids and cholesterol. The ratio and type of these lipids are critical for determining the liposome's rigidity, stability, and permeability.
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Particle Size: Liposomes typically range from 20 nm to 1000 nm in diameter. Precise size control is essential, as it directly impacts biodistribution, circulation time, and cellular uptake. For example, smaller liposomes can more easily cross certain biological barriers.
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Potential (Zeta Potential): The Zeta potential measures the surface charge of the liposome. A repulsive charge helps prevent particles from aggregating, ensuring stability. The surface charge also plays a critical role in cellular interactions and uptake, with cationic (positive) liposomes often used for nucleic acid delivery.
Why Choose Liposome-Based Drug Delivery?
Liposomes offer a versatile solution to many of the most persistent challenges in drug development. Their inherent properties provide significant advantages that can be leveraged to improve therapeutic outcomes.
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Improved Solubility: Enables delivery of poorly soluble compounds by encapsulating both hydrophilic and hydrophobic drugs.
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Enhanced Stability: Protects payloads from enzymatic degradation and harsh environments, ensuring drug activity until it reaches the target.
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Reduced Toxicity: Increases drug concentration at the target site while reducing exposure to healthy tissues, minimizing side effects.
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Biocompatibility: Composed of natural, biodegradable lipids, they are generally non-toxic for in vivo use.
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Targeting Capabilities: Allows for precise, site-specific delivery via modification with targeting modules.
The Payloads We Deliver
Our liposome technology platform is designed for the effective encapsulation and delivery of a broad range of therapeutic and bioactive molecules. We handle the most challenging payloads with precision and care.
Small Molecule Drugs
Chemotherapeutics, immunosuppressants, anti-inflammatory agents, and more.
Nucleic Acids
siRNA, shRNA, mRNA, miRNA, ASOs, DNA plasmids, and advanced RNA-guided gene editing systems.
Proteins & Peptides
Therapeutic enzymes, antibodies, antibody fragments (scFv, Fab), signaling peptides, hormones, and vaccines.
Advanced Therapeutics
Protein degraders, Vitamins, diagnostics, contrast agents, and various biomolecules requiring targeted delivery.
Our One-Stop Liposome Development Services
Learn More about Liposome Development Services
Creative Biolabs provides a complete, one-stop platform for all your liposome needs. By offering an integrated workflow from synthesis to validation, we ensure seamless project progression and superior results.
Chemical Synthesis
We provide comprehensive chemical synthesis services to support the creation of custom liposome formulations. Our capabilities extend from the synthesis of novel lipids to the modification and derivatization of various therapeutic payloads, ensuring you have the precise molecular building blocks required for your research.
Advantages
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Novel lipid and derivative access.
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Precise payload modification.
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Scalable synthesis (mg to kg).
Quality Control (QC)
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Purity analysis.
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Structural confirmation
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Batch-to-batch consistency
Liposome Development
Our team specializes in the development of custom liposome formulations to meet the specific requirements of your research.
Charged Liposomes
Tailored formulations for neutral, cationic, and anionic liposomes to optimize interactions with target cells and nucleic acids.
Fluorescent Liposomes
Production of fluorescently labeled liposomes for in vitro imaging, cell tracking, and biodistribution studies.
Drug-Loaded Liposomes
Expert encapsulation of a wide array of payloads, from small molecules to proteins, into liposomal carriers.
Long-Circulating Liposome Design
Engineering of long-circulating liposomes (e.g., PEGylated liposomes) to enhance their systemic residence time and improve drug delivery to a target site.
Functional Liposome Development
We integrate our extensive targeted modules to assist clients in developing advanced functional liposomes, all engineered to achieve highly specific targeted delivery.
Immunoliposomes (Antibody-conjugated)
Peptide-Conjugated Liposomes
Aptamer-Conjugated Liposomes
Development of Novel Liposomes
At Creative Biolabs, we stay at the forefront of the field by specializing in the development of cutting-edge liposome technologies. Our novel formulations are designed to overcome the limitations of traditional systems and enable next-generation therapeutic applications.
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Cell Membrane-Coated Liposomes
These biomimetic vesicles are engineered to evade the immune system and enhance targeting. By using membranes from specific cells, they can mimic natural cell properties for improved delivery.
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Macrophages
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Dendritic Cells
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Immune Cells
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Tumor Cells
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Red Blood Cells
We combine the natural targeting and low immunogenicity of exosomes with the scalability and customizability of liposomes to create highly potent delivery systems.
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Natural Targeting (from exosomes)
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Low Immunogenicity (from exosomes)
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Scalability (from liposomes)
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Customizability (from liposomes)
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Stimuli-Responsive Liposomes
We design advanced stimuli-responsive liposomes that release their payload in response to specific triggers, enabling precise, localized delivery. These triggers can include:
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Exosome-Mimicked Liposomes
We design liposomes that closely mimic the essential properties of natural exosomes. By carefully adjusting lipid composition and ratios, we create synthetic mimics that serve as reliable controls, ensuring robust and reproducible experimental results. Leveraging these features, we also deliver scalable systems that capture the advanced functionality of exosomes—empowering research in gene therapy and intercellular communication.
Liposome Characterization
At Creative Biolabs, we provide a full suite of comprehensive liposome characterization services to ensure the quality, consistency, and performance of your formulation. Our rigorous analysis confirms that your liposomes meet the highest standards for research and development. We offer:
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DLS Analysis: In-depth Dynamic Light Scattering (DLS) analysis to determine particle size and polydispersity index (PDI).
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Encapsulation Efficiency: Precise measurement of encapsulation efficiency to quantify the percentage of payload successfully loaded into the liposomes.
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Release Kinetics: Study of drug release profiles to understand how the payload is released over time under various conditions.
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Stability Testing: Comprehensive stability studies to assess the physical and chemical integrity of the liposomes over time.
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Safety Assessment: Evaluation of the liposome formulation for cytotoxicity and biocompatibility.
Liposome Validation Services
We offer both in vivo and in vitro validation services, leveraging our extensive collection of cell and animal models to assist clients in verifying the biological distribution, targeting, and efficacy of their liposome formulations.
Large-Scale Production
We offer large-scale production services for scientific research, capable of manufacturing from mg-level to kilogram-level quantities to support projects from initial feasibility studies to large-scale preclinical trials.
Workflow
Applications of Liposome Technology in Modern Research
Liposomes are a versatile platform with a wide array of applications across various research fields, providing a pathway for the delivery of diverse therapeutic payloads.
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Drug Delivery Systems: Encapsulating small molecule drugs, such as chemotherapeutics and anti-inflammatory agents, to improve solubility and reduce systemic toxicity.
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Gene Therapy: As a premier non-viral vector, liposomes provide a safe and efficient means to deliver a suite of nucleic acids—including siRNA, shRNA, mRNA, and RNA-guided gene editing systems components. This enables precise manipulation of cellular function, from targeted gene silencing and transient protein expression to sophisticated gene editing.
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Vaccine Development: Liposomes act as powerful adjuvants and delivery systems in next-generation vaccines. By enhancing antigen presentation and promoting its uptake by immune cells, they elicit a stronger, more durable, and targeted adaptive immune response.
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Cancer Research: Utilizing targeted liposomes to deliver payloads directly to tumor sites, improving efficacy while sparing healthy cells.
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Diagnostic Imaging: Encapsulating contrast agents and other diagnostic molecules to improve resolution and specificity in imaging techniques.
Why Partner with Creative Biolabs?
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Choosing Creative Biolabs means choosing a partner committed to scientific excellence and collaborative success.
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Unmatched Expertise: Our team isn't just experienced; we are pioneers in the field of advanced drug delivery. With over 20 years dedicated to mastering liposome technology, we possess the nuanced understanding to tackle your most complex challenges.
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Holistic Approach: Our unique strategy combines foundational Module Delivery Systems (liposomes) with sophisticated Targeted Modules (aptamers, peptides), creating truly innovative solutions tailored to your needs.
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Comprehensive Service: From the initial concept and rational design to meticulous preparation, characterization, and final formulation, we manage the entire workflow. This comprehensive, one-stop-shop service eliminates the need to coordinate between multiple vendors, saving you invaluable time and resources while ensuring consistency and quality at every stage.
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Rigorous QC: Every liposome batch undergoes comprehensive physicochemical characterization, including size, polydispersity, Zeta potential, and encapsulation efficiency, ensuring peak performance and reproducibility for your experiments.
Ready to overcome your drug delivery challenges and accelerate your research? We provide the high-quality, customized liposome solutions you need to bring your scientific vision to life.
Related Services
Related Products
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Empty Liposome
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A blank liposome formulation (no payload) for use as a control in your experiments.
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Clodronate Liposome
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Clodronate liposomes are a specialized type of liposome designed to selectively deplete macrophages. They are essentially liposomes that encapsulate the bisphosphonate drug clodronate.
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DiD-labeled Fluorescent Liposomes
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Optimized for in vivo imaging with a near-infrared fluorescent dye (DiD) to track liposome biodistribution.
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Exosome-Mimicked Liposomes
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Engineered to mimic the surface properties of natural exosomes, offering enhanced targeting and low immunogenicity.
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FAQs
What types of payloads can be encapsulated in liposomes?
We can encapsulate a wide variety of payloads, including small molecule drugs, nucleic acids (mRNA, siRNA, DNA plasmids), proteins, peptides, and advanced therapeutics like protein degraders.
How do you ensure targeted delivery?
We achieve targeted delivery by functionalizing the liposome surface with specific ligands, such as antibodies or peptides. This active targeting mechanism allows the liposomes to bind to specific receptors on target cells or tissues.
What is the typical turnaround time for a customized service?
Turnaround time varies depending on the complexity of the project, including the payload type, required scale, and formulation specifications. We will provide a detailed timeline after an initial consultation to fully understand your requirements.
What QC measures do you perform on the final product?
Our QC process includes a comprehensive analysis of liposome size, polydispersity index (PDI), zeta potential, and encapsulation efficiency to ensure the product meets your specifications.
Can I provide my own lipids or payload for the service?
Yes, we can work with your proprietary lipids or therapeutic payloads. Our customized services are designed to be flexible and integrate seamlessly with your existing research materials and goals.
Reference
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Rommasi, Foad, and Neda Esfandiari. "Liposomal nanomedicine: applications for drug delivery in cancer therapy." Nanoscale research letters 16.1 (2021): 95. doi:10.1186/s11671-021-03553-8. Distributed under an Open Access license CC BY 4.0, without modification.
Our services are For Research Use Only. We do not provide services to individuals.
