NGS Sequencing

Next generation sequencing (NGS) technology offers a giant leap forward for the future of vaccinology. With advances in the current technology and new single-molecule sequencing technologies on the horizon, it will become cost and time efficient. Advances in the analysis of such data may well revolutionize how we practice vaccinology. Creative Biolabs have been specialized in biomarker discovery and data analysis services, including highly professional knowledge, especially for NGS Sequencing.

NGS Sequencing

As a high-throughput sequencing technology, the NGS sequencing has redefined the research of vaccine technology, allowing a very large sequencing data set to be generated at an affordable cost in a short period of time and at affordable costs. Vaccine development research is rapidly taking full advantage of the emergence of NGS. NGS research helps to develop vaccines for human infectious diseases, including both pathogens and hosts. NGS unlocks the complexities of virus evolution and host response to these viruses, greatly contributing to the potential for successful vaccine development. Creative Biolabs focuses on the rapid mutation of viral pathogens, which is one of the main targets of the current vaccine research.

Our Services

• Reversible terminator sequencing (Illumina): The Illumina / Solexa Genome Analyzer involves sequencing sequencing-by-synthesis using reversible terminators. Fragmented ssDNA is hybridized to oligonucleotide anchoreds on a solid surface called "flow cell". Solid phase bridge amplification of DNA templates is performed to generate expanded clusters. Massively parallel sequencing of cleavage products from amplified clusters is performed using a DNA polymerase and a set of four base-specific color-coded reversible terminators that leads to growth. The platform initially produced 35-bp reads to yield 1 Gb of sequence output every 2-3 days. Subsequent upgrades on this platform have added cluster density and read length, allowing the machine to produce 4 Gb of sequence output per 2–3-day run;
• 454 Pyrosequencing: The 454 method can sequence fragments of DNA equivalent to up to one billion bases in a single day, which is much cheaper and faster than the other methods. But the platform also has some disadvantages such as having difficulty distinguishing the number of bases in a run of identical bases;
• Ion torrent semiconductor sequencing: Semiconductor-based device is based on emPCR, SBS and “ion detection (pH metre that sequences)” of protons released as dNTPs are incorporated during synthesis, rather than on dye-labellingand potical imaging. DNA to be sequenced is bead-captured and settled in microwells, and unmodified dNTPs are floated across the wells, one at a time. The polymerase incorporates the appropriate dNTPs into the growing strand, and the H⁺ ion is released changes the pH in the solution, which can be detected by a hypersensitive ion sensor layer beneath each microwell in real time. If homopolymeric sequences are present, multiple nucleotides are incorporated during each cycle, leading to the release of a corresponding number of ions and proportionally higher electronic signal;
• Sequencing by ligation (SOLiD): The size of the beads of the initial emulsion for PCR are only 1 μm. The amplified product on the beads are covalently attached to the glass surface and sequencing is carried out using hybridized-ligated with an octamer interrogation probe consisting of two probe specific, three degenerate and three promiscuous bases. Each nucleotide position is determined using a four-dye coding mode, and each position is interrogated twice to distinguish sequencing errors from single nucleotide polymorphisms (SNPs).

NGS has allowed us to sequester DNA and RNA faster and cheaper than ever before. These latest technologies not only improve accuracy and speed but also reduce manpower and costs, which have revolutionized genomics and molecular biology research. Creative Biolabs is an expert company for vaccine targeting discovery services, including high expertise in next-generation-sequencing (NGS). Our scientists with years of experiences in NGS methodologies, coupled with advanced infrastructure, enable us to work effectively with our customers.

All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.