Suspension Culture for Pseudorabies Vaccine Production

Creative Biolabs optimizes the complete vaccine production system and provides comprehensive media development services for vaccine production. We offer formulations for cell lines that act as hosts for viral production. Count on us to help address your challenges and get your process going.

Pseudorabies Vaccine – Creative Biolabs Pseudorabies Virus (PRV)

Pseudorabies virus (PRV) is a double-stranded, DNA-based swine virus with a genome approximating 150 kb in size. Pseudorabies, also known as Aujeszky's disease, is caused by Suid herpesvirus 1 (SuHV1). It is a viral disease of pigs that has been endemic in most parts of the world and has caused substantial economic losses to swine producers. Infection is usually fatal in susceptible animals other than swine, such as dogs, cats, cattle, goats, sheep, and raccoons, are also susceptible. Research on SuHV1 in pigs has pioneered animal disease control with genetically modified vaccines. SuHV1 is now used in model studies of basic processes during lytic herpesvirus infection, and for unravelling molecular mechanisms of herpesvirus neurotropism.

Vaccines against Pseudorabies Virus

Although no specific treatment for acute infection with SuHV1 is available, vaccination can alleviate clinical signs in pigs of certain ages. Aujeszky´s disease is considered a blueprint for the use of efficacious inactivated and attenuated vaccines as well as for trend-setting innovations and ground-breaking technologies for the development of highly potent and safe genetically engineered marker vaccines. Typically, mass vaccination of all pigs on the farm with a modified live virus vaccine is recommended. Both live and inactivated vaccines against PRV infections were primarily developed for reducing clinical signs upon infection or for disease prevention to minimize the associated economic losses in swine. Evidence that vaccination can reduce shedding of attenuated and virulent PRV in swine and transmission to susceptible in-contact animals has been documented.

Shortcomings of Traditional Pseudorabies Vaccine Production

Swine testis (ST) cells are regarded as one of cell lines most suitable for producing vaccines against pseudorabies virus. At present, a micro-carrier adherent culture production method utilizing an ST cell line to culture the pseudorabies virus has already been developed. Although the technological method can reach certain production scale, it still has some defects:

  • Micro-carriers are difficult to reuse repeatedly, leading to high production cost;
  • The culture density of adherent cells is limited by the adherent area, resulting in low yield of the viruses;
  • The adherent culture generally needs serum to help the attachment and growth of the cells and needs to change the media, so the process is complicated. Mycoplasma, chlamydia or animal protein may be introduced to cause the pollution, thereby posing a potential threat to the safety of vaccine products.

Suspension Culture for Pseudorabies Vaccine Production

In view of the deficiencies of the traditional methods, Creative Biolabs is developing a low serum media for full suspension culture of ST cells. The media supports the high-density full suspension culture of the ST single cells, greatly shortening the time for educating the ST cells from the serum adherent cultured cells to the serum-free full suspension cultured cells and is applicable to the mass production of biological products and particularly the veterinary biological products, including vaccines against pseudorabies virus.

Features
Advantages
  • Full suspension for seed batches, expansion and manufacturing;
  • Low cost: low serum, no micro-carriers, low labor cost;
  • Low serum (1-2%);
  • Easy to operate with high stability: no need to change media;
  • Exclusive ST suspension cell line;
  • Low side effects: low impure protein;
  • Exclusive suspension media;
  • High yield: Viral titer is 5-10 times higher than roller bottle culture;
  • Suitable for a variety of vaccine strains including Bartha-61 and HB98.
  • Suitable for large-scale production: automated bioreactor.

In Creative Biolabs, low serum ST cell culture media has been optimized for the growth and expansion of ST cells under low serum (1-2%) conditions in suspension culture. This media has been tested for its ability to support high-density suspension cultures of ST cells adapted from adherent culture platforms. This is a complete media that will support growth of ST cells without further supplementation. Other cytokines and growth factors may be supplemented to facilitate specific cell applications. Please feel free to contact us for more details.

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