Suspension Culture for Rabies Vaccine Production

Creative Biolabs optimizes the complete vaccine production system and provides comprehensive media development services for vaccine production. We offer formulations for cell lines that act as hosts for viral production. Count on us to help address your challenges and get your process going.

Rabies Virus

Rabies virus, which has a bullet like shape with a length of about 180 nm, is a neurotropic virus that causes rabies in humans and animals. It is the type species of the Lyssavirus genus of the Rhabdoviridae family, all of which consist of two major structural components: a helical ribonucleoprotein core (RNP) and a surrounding envelope. The rabies genome encodes five proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and polymerase (L). Rabies virus has an extremely wide host range, from mammalian species to cell cultures from mammals, birds, reptiles, and insects. The spread of rabies can occur through the animal's saliva and less commonly through contact with human saliva.

Rabies virus – Creative Biolabs.

Rabies Vaccines

Virtually all infections with rabies resulted in death. Rabies vaccine is a vaccine used to prevent rabies, which was first developed by two French scientists, Louis Pasteur and Émile Roux. They collected a sample of the virus harvested from infected rabbits and weaken the virus by allowing it to dry for 5 to 10 days. The human diploid cell rabies vaccine (H.D.C.V.) was started in 1967, using the attenuated Pitman-Moore L503 strain of the virus. Other vaccines include purified chicken embryo cell vaccine and purified Vero cell rabies vaccine. Rabies vaccine can be used to prevent rabies before and for a period of time after exposure to the virus such as by a dog or bat bite. The immunity that develops is long lasting after a full course.

Serum-Free Suspension BHK Cell Line Culture for Avian Influenza Vaccine Production

Rabies virus – Creative Biolabs.

Very few cell lines are currently used for the production of rabies vaccines for veterinary use: NIL2 and BHK-21. Generally, BHK-21 Cl3 cells are grown as surface adherent cells in media containing serum (SCM) which may be replaced by bovine serum albumin (BSA). SCM have several disadvantages:

  • Sera vary in quality and composition between batches and producers
  • High quality sera are particularly expensive
  • May also be contaminated by microorganisms (bacteria, mycoplasma, fungi, viruses, the BSE agent, etc.).

Satisfactory serum-free medium would be very useful for developing a less expensive bioprocess for biologicals as well as for avoiding many of the problems of variability and contamination associated with sera. Several serum-free medium have been developed in Creative Biolabs and are currently used for the production of inactivated vaccines against rabies virus.

Advantages

There are many advantages of serum-free suspension cell cultures over monolayer cultures.

  • It is not necessary to trypsinize cells in suspension culture, thereby reducing the possibility of deleterious effects on both the cells and on infectious agents.
  • BHK cells cultivated in shaker cultures do not clump readily. Sampling, therefore, becomes simplified and more reproducible.
  • Less medium is used to produce the same number of cells.
  • Reducing the chance of microbial contamination.
  • Low cost: low serum, no micro-carriers, low labor cost.
  • Easy to operate with high stability: reducing the burden of downstream purification process.
  • High antigen content: completely meet the requirements for the semi-finished product of the antigen.
  • Suitable for large-scale production.

Features

  • Full suspension culture medium;
  • Serum-free culture;
  • Inactivated rabies vaccine production;
  • Suitable for a variety of strains such as PV, Flury, SAD, and aG.

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