The Purity Tests of Vaccines
The vaccine plays an increasingly important role in disease prevention. In recent years, the application of advanced separation and purification techniques in the preparation of traditional and new vaccines is an effective means to improve the efficacy of vaccines and reduce side effects. Creative Biolabs is a world leader in the field of vaccine analytical development. With our extensive experience and advanced platform, we are therefore confident in offering the best development services for the purity test of vaccines. We guarantee the finest results for our customers all over the world.
Fig.1 The Development Process of Flu Vaccines.
The Vaccine Purification Route
There are great or small differences in the development method, composition, physical, chemical properties and original source of the vaccine. Different methods of isolation and purification should be used for different vaccines, but generally, there are two basic stages: primary and specific isolation and purification. The main tasks of the primary stage of separation are to separate cells and culture media, crush cells to release products (if products are in cells), concentrate products and remove most impurities. In the stage of specific purification, various technologies with high resolution are selected to separate the target protein and a small amount of interfering impurities as far as possible, and the required quality standards are met. Ultra-rapid centrifugation and various chromatography technologies have become the main methods to achieve this goal.
The Membrane and The Speeding Centrifuge Technologies
The membrane separation and speeding centrifuge technologies have a promising future in the process of the purification of vaccines, proteins, polypeptide and biological molecules. The purity and properties of the vaccine prepared by this process meet the requirements of the procedures, but the recovery rate of antigens is relatively low, the operation is tedious, the cycle is long and the technical equipment is high. In recent years, it has been gradually replaced by the increasingly mature tomography technology, especially in the development of genetic engineering vaccine.
The Chromatographic Separation Technology
The chromatographic separation technology shows an increasing advantage in simplifying operation, improving efficiency, saving energy, and reducing cost. The introduction of chromatographic media with multifunctional ligands and a fully automatic, efficient chromatographic system has expanded application and is more convenient for standardization implementation. Affinity chromatography and ion exchange chromatography may be the most effective methods for the purification of the target protein from low concentration medium.
Our Strategies to Test the Purity of Vaccines
Our purification technology for traditional and new vaccines are developed by the combination of related technologies of various disciplines. Up to now, no single technology can be used to undertake the whole process of isolation and purification. Creative Biolabs’ innovative separation and purification process is often composed of several new technologies and the advantages of the original technology. Traditional centrifugation, filtration and precipitation technologies are mainly used as the starting step of the whole separation and purification process for preliminary separation. The combination of chromatography with precipitation, centrifugation, and other traditional separation technologies has gradually become the main method of our vaccine separation and purification.
- High resolution and a more uniform particle size distribution
- High recovery rate and the high cost performance
- Extremely high-pressure resistance and a high flow rate
- High load, high velocity, and high recovery
Creative Biolabs is a leader in the field of vaccine development and has focused on the vaccines analytical development and qualification services for years. We have experienced experts and advanced platforms that are able to provide excellent services. If you are interested in our services, please contact us for more details.
Reference
- Scott, P. (2016). “Vaccine provision: Delivering sustained & widespread use.” Vaccine 34(52), 6665-6671.
All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.
