Vibrio Anguillarum as Vaccine-vectors

Nowadays, virulence-attenuated pathogens are considered to be powerful vectors for heterologous antigens to be used in prophylaxis and therapy because of their unique advantages. In recent years, Creative Biolabs is dedicated to the research and design of bacteria as an antigen delivery vehicle, such as Vibrio anguillarum. As a service provider, our goal is to provide the highest level of service, confidentiality and customer support.

Vibrio Anguillarum as Delivery Vehicle

Vibrio anguillarum as Vaccine-vectors– Creative Biolabs

Vibrio anguillarum (V. anguillarum), a Gram-negative bacterium, is an important marine fish pathogen responsible for both marine and fresh-water epizootics, causing fatal haemorrhagic septicaemia in salmonids and other fish. In recent years, living bacterial vectors have received increasing attention as gene or protein delivery systems, and V. anguillarum has also been developed as a foreign protein delivery vehicle. Several attenuated V. anguillarum strains derived from wild-type V. anguillarum strain MVM425 had been constructed by recombinant DNA technology and proved to be excellent live vaccine candidates against Vibrio pathogens. For developing potential multivalent recombinant vaccines based on attenuated V. anguillarum, it is necessary to effectively display the protective antigen on its surface or to express it efficiently and stably.

Our Strategies of Vibrio Anguillarum as Vaccine-vectors

  • Strains

The strain MVAV6203 with the curing of virulence-related plasmid pEIB1 and unmarked gene deletion of the aroC was attenuated by 10,000 times and was shown to confer excellent immune protection against Vibrio pathogens. Therefore, MVAV6203 is considered to be a viable candidate for V. anguillarum as a live vaccine vector.

  • Plasmid expression system

In the design of bacterial vector vaccines, the level and persistence of antigen expression in the host are essential for eliciting a protective immune response, and plasmid stability appears to have a substantial effect on the long-lasting expression of the heterologous protein. In order to increase the level and persistence of antigen expression in attenuated V. Anguillarum strain (MVAV6203), thereby enhancing the induced immune response, the design of a suitable attenuated bacterial plasmid vector system is critical. We design the plasmid pUTat, which was engineered by replacing the replicon of pUTt plasmid with the replicons derived from pAT153 that showed the best stability in plasmid retention and protein expression both in vitro and in vivo.

  • Surface display system

In order to develop potential multivalent recombinant vaccines based on attenuated live V. anguillarum, an efficient surface display system is needed to display protective antigens on the surface of live vectors. Using V. anguillarum outer membrane proteins and outer membrane lipoproteins as anchoring motifs, Creative Biolabs offers a range of novel cell surface display systems. These display systems include (i) the signal sequence and first 11 N-terminal amino acids of V. anguillarum outer membrane lipoprotein Wza, or the signal sequence and first 9 N-terminal amino acids of the mature major Escherichia coli lipoprotein Lpp, and (ii) transmembrane domains of the outer membrane protein Omporf1, OmpU or Omp26La of V. anguillarum.

With years of experience and advanced vaccine technology platform, Creative Biolabs has developed engineered and attenuated V. anguillarum vectors that deliver proteins in vitro and in vivo. If you have any need for V. anguillarum as a delivery vehicle, we are your best choice.


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