Phage Display HuScL-6 Human Antibody Library Screening Service

Basic Information Features Library Screening Cases Related Libraries Why Work with Us?

The HuScL-6 Human Single Chain Antibody Library is a state-of-the-art resource for efficient, high-quality antibody screening. Designed to meet the diverse needs of researchers, this library provides access to a rich pool of scFv antibodies derived from human naïve antibody genes. With our >90% success rate in protein target screening and strong capabilities for screening cell-based and other complex targets, we are confident that our library can deliver exactly what you need to advance your research.

Get Your Custom Quote Today!

Basic Library Information

Species	Human	Library ID	HuScL-6
Display Technology	pIII-fusion, Phagemid phage display system	Library Format	VH-VL format scFv antibody library
Vector System	pCANTAB 5E	Tags	E tag
Library Capacity	2.1×10¹¹	Library Source	Human antibody genes from 4000 naïve donors
Host Strain	TG1 cells for proliferation HB2151 cells for antibody production	Helper Phage	M13KO7

Why Choose the HuScL-6 Library?

Exceptional Success Rates

Our library ensures dependable high-quality research outcomes with over 90% protein target screening success and more than 80% success across cell-based and various other target types. This ensures a high yield of functional antibodies, even for challenging targets.

Comprehensive Screening Options

Our library accommodates numerous target types including cell-based models, peptides, small molecules and toxins while delivering customized solutions designed to fulfill your research requirements.

Diverse Human Antibody Source

Sourced from over 4,000 human naïve antibody donors, the HuScL-6 library offers an extensive and diverse selection of antibodies, significantly increasing the chances of finding the ideal match for your target.

Scalable & Efficient Screening

With a vast library capacity of 2.1×10¹¹, we provide both small- and large-scale screening capabilities, ensuring quick and reliable results without compromising on quality.

Want to Know More? Get a Quote Today!

High-Efficiency Screening for Precision Research

Available Screening Service Types

We offer a comprehensive range of screening services for various target types, ensuring we can meet the diverse needs of your research projects. Our screening capabilities include, but are not limited to, successful projects we have previously undertaken in the following areas:

Antibody Types:
Bispecific Antibodies	Anti-Idiotype Antibodies	Anti-Epitope Antibodies	Cell-Internalizing Antibodies
Target Types:
Membrane Protein Targets	Cell-based Targets	Enzyme Inhibitors	MHC-Peptide Complexes
Disease and Biomarker Targets:	Viral Antigens	Cancer Antigens	Receptor Targets
Small Molecule Targets	Toxin Targets

How It Works: Our Screening Process

Submit Your Target

Provide your target information (protein, peptide, small molecule, cell-based, etc.) and any specific requirements.

Screening Process

We use our HuScL-6 library to perform antibody screening using phage display technology. This step ensures rapid identification of high-affinity antibodies.

Results Delivery

After screening, we provide you with a detailed report, including the best candidate sequences and their binding affinity and specificity.

Support and Guidance

Our team stands ready to help address your questions and analysis needs throughout the process.

Note: We provide screening-only services, and do not offer enriched libraries after multiple rounds of screening. Our focus is on providing you with high-quality screening results for your specific targets.

Discover Your Solution - Request a Quote!

Case Studies: Real Results from Our Screening Services

Case: Screening for Anti-Type 1 Idiotype Antibody
Starting Materials	Target biotinylated IgG11 Control isotype control IgG1 Antigen 1
In-Solution Screening Strategy	Pre-counter selection using isotype control IgG Positive selection for biotinylated Target IgG Competitive screening using isotype control IgG1 to further remove the binders that are bound to the constant regions and Framework regions. Elution with antigen.
Key Results	After conducting four rounds of screening and validating 92 randomly cloned phages through ELISA, we successfully obtained nine candidate clones that specifically target the antigen. These candidates were further validated for their anti-idiotype properties using soluble competitive ELISA techniques.

Case: Screening for MHC-Peptide Complex Target
Starting Materials	Biotinylated target MHC-peptide 1 Biotinylated control MHC-peptide Control MHC-peptide
In-Solution Screening Strategy	Pre-counter selection using Biotinylated control MHC-peptide Positive selection for biotinylated Target MHC-peptide, control MHC-peptide was used as competitor to further remove the binders that were bound to the control MHC-peptide.
Key Results	We conducted four rounds of screening targeting the MHC-peptide. After identification through monoclonal phage ELISA, we obtained seven positive clones. These clones were further validated for their binding capacity using soluble ELISA. All seven clones exhibited specific bindings to the target MHC-peptide and did not bind to the control MHC-peptide.

Case: Screening for Cell-Internalizing Antibody
Starting Materials	Target cell T1: one type of primary human cell Control cell N1: a mixture of several types of primary human cells
Cell-based Screening Strategy	Pre-counter selection using control cell N1 Positive selection for target cell T1 Phage FACS for random clones against target cell T1 Soluble ELISA and soluble FACS against target cell T1 NGS for enriched library outputs
Key Results	We screened antibodies that can specifically bind to cell-internalizing targets using phage display technology. The internalization process was validated through flow cytometry, demonstrating that most screened antibodies were able to efficiently enter the target cells.

Explore Other Available Premade Human Antibody Libraries

Species	Antibody Library ID	Display Technology	Library Format	Library Size	Out-licensing
Human	HuScL-6	pIII-fusion, Phagemid Phage Display	Naïve scFv	2.1×10¹¹	Screening-only
	HuScL-7	pIII-fusion, Phagemid Phage Display	Naïve scFv	1.1×10¹⁰	✔️
	HuScL-3S	pIII-fusion, Phagemid Phage Display	Semi-synthetic scFv	>1.0×10¹¹	Screening-only
	HuFabL-4	pIII-fusion, Phagemid Phage Display	Naïve Fab	1.9×10¹⁰	Screening-only
	HuFabL-5	pIII-fusion, Phagemid Phage Display	Naïve Fab	1.1×10¹⁰	✔️
	HuFabssL-1	pIII-fusion, Phagemid Phage Display	Naïve & synthetic Fab	1.8×10¹⁰	Screening-only
	HuscL-YDP-3	Yeast Display	Naïve scFv	1.9×10⁸	✔️

Why Work with Us?

Proven Expertise

We specialize in antibody screening, delivering highly accurate and diverse antibody candidates. With a proven success rate of >90% for protein targets, we can help you identify the perfect antibody for your project.

Cutting-Edge Technology

Our use of phagemid phage display technology ensures fast and efficient screening, giving you the results you need, when you need them.

Broad Target Capability

Whether your target is a cell-internalizing antibody, an MHC-peptide complex, or a blocking antibody, we have the tools and expertise to handle your unique screening needs.

Need more details? Please feel free to ask any questions and we will adjust our services to perfectly match your requirements. Get in touch today!

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: