Hamster IL1B ELISA Kit, Lot 21AO-283 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Hamster IL1B in Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate.

For the quantitative determination of hamster interleukin 1beta (IL-1beta) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

ELISA

The supplier is only responsible for the kit itself, but not for the samples consumed during the assay.
Please predict the concentration before assaying.
If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits, some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
Fresh samples without long time storage are recommended for the test.

Detection wavelength: 450 nm
Information on standard material: Depending on the antigen to be detected, standards can be either native or recombinant protein.
Information on reagents: In most cases the stop solution provided is 1 N H2SO4.
Information on antibodies: The antibodies provided in different kits vary in regards to clonality and host.

IL1B

Hamster

Colorimetric

Sandwich ELISA

Quantitative

Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate

This assay has high sensitivity and excellent specificity for detection of hamster IL-1beta.

Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Assay plate (12 × 8 coated Microwells)
Standard (freeze dried)
Biotin-antibody (100 × concentrate)
HRP-avidin (100 × concentrate)
Biotin-antibody Diluent
HRP-avidin Diluent
Sample Diluent
Wash Buffer (25 × concentrate)
TMB Substrate
Stop Solution
Adhesive Strip (for 96 wells)
Instruction manual

Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
Squirt bottle, manifold dispenser or automated microplate washer.
Absorbent paper for blotting the microtiter plate.
100mL and 500mL graduated cylinders.
Deionized or distilled water.
Pipettes and pipette tips.
Test tubes for dilution.

3.12 pg/mL

100 μL

1 - 4.5 h

Pre-coated

Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit.

Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
Intra-assay: CV% less than 8%
Inter-assay: CV% less than 10%

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

1.All reagents must be at room temperature (18 °C to 25 °C) before running assay.
2.Do not expose kit reagents to strong light during storage or incubation.
3.Improper or insufficient washing at any stage of the procedure will result in either false positive or false negative results.
4.Avoid contact of stop solution with skin or eyes. If contact occurs, immediately flush area with copious amounts of water.
5.Do not use TMB substance solution if it has begun to turn blue.
6.Do not expose bleach to work area during actual test procedure because of potential interference with enzyme activity.

4 °C/-20 °C

May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.

6 months

May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.

For Research Use only

Interleukin 1 Beta (IL1b)

IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b

NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome

Antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step.