High-Content Multiplexed Cell & Bead-Based Detection Service

The Overview of High-Content Multiplexed Cell & Bead-Based Detection Service

Antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in drug development. Screening high-affinity antibody candidates directed against cell-surface antigens is a crucial step in discovering therapeutic antibodies. In recent years, cell and bead-based flow cytometry assays have been shown to be an interesting option for multiplex quantification and detection of analytes. Thus, developing a high-content multiplexed cell & bead-based detection service has great significance in boosting drug development of cancer therapy.

Fig.1 Scheme of main therapeutic antibody formats developed for the treatment of the disease. (Lu, et al., 2020)

Our High-Content Multiplexed Cell & Bead-Based Detection Service

Creative Biolabs is committed to providing a rapid and effective high-content multiplexed cell & bead-based detection service based on the principle that antibody-conjugated multiplexed beads or cells can quantitatively detect an enormous number of analytes with high affinity. Each bead or reporter cell in the assay has been labeled with unique fluorescence, which is allowed precisely binding to cell surfaces, and combining different analytes in the same well with multiple fluorophores to identify separate markers and reject background fluorescence due to media or fluorophores.

Fig.2 Dual-encoded bead-based immunoassay formats. (Lin, et al., 2022)

Published Data

Here are some representative published data displays.

Cell-based

Representative data 1: Multiplex cell-based immunoassay application in domain/epitope mapping using antibodies specific for human CCR2 and CCR5. The results showed that pooled reporter cell lines are easy to screen with flow cytometry and facilitate existing antibody isolation techniques including hybridoma, single-cell PCR, and single B-cell cultures to aid in antibody discovery and engineering.

Fig.3 Multiplex cell-based immunoassay application in domain/epitope mapping. (Song, et al., 2021)

Bead-based

Representative data 2: Multiplexed CBA (cytokine bead array) applied in identifying a dot plot for the five recombinant cytokines. The results showed that native cytokines from cell culture supernatants were tested using beads and flow cytometry has a wider dynamic range.

Fig.4 Multiplexed CBA (cytokine bead array) application in identifying cytokines. (Rodrigues, etal., 2017)

Work with Creative Biolabs

Creative Biolabs provides a top-ranking high-content multiplexed cell & bead-based detection service. Our competent team, together with our diverse service portfolio, gives us confidence in providing high-quality data in a timely manner.

If you are interested in our high-content multiplexed cell & bead-based detection service, please feel free to contact us. We are sincerely looking forward to working with global customers.

References

1. Lu, R.M.; et al. Development of therapeutic antibodies for the treatment of diseases. J Biomed Sci. 2020, 27(1):1.
2. Lin, Z.; et al. Dual-encoded bead-based immunoassay with tunable detection range for COVID-19 serum evaluation. Angew Chem Int Ed Engl. 2022, 61(37): e202203706.
3. Song, S.; et al. A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines. Commun Biol. 2021, 4(1):1338.
4. Rodrigues, V.; et al. Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry. Cytometry A. 2017, 91(9):901-907.

For Research Use Only | Not For Clinical Use