Hbl-2 In Vitro RNA Expression Analysis Assay (DNA Microarray)

This assay is to provide Hbl-2-based In Vitro RNA Expression Analysis Assay (DNA Microarray) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Hbl-2

Human

The HBL-2 cell line is derived from an AIDS-SNCCL (AIDS-associated small non-cleaved cell lymphoma) patient. After immunophenotypic and molecular genetic analysis, the HBL-2 was established from the original tumor clones. The HBL-2 cell line presents surface immunoglobulin and B-cell restricted markers as well as a phenotype consistent with SNCCL; the cell line also displays clonal immunoglobulin gene rearrangement. The HBL-2 cell line is considered useful as a biological model to study AIDS-associated non-Hodgkin lymphomagenesis (AIDS-NHL) and the impacts of biological, immunological, and viral factors involved.

Mantle Cell Lymphoma; MCL

Oncology

In Vitro RNA Expression Analysis Assay (DNA Microarray)

DNA microarray is another technique that enables the analysis of gene expression. Fluorescent labeled cDNA (with cyanines) synthesized from a test sample is hybridized to target probes with complementary sequences attached on microarray chips and strongly bound complexes are screened after scanning optically.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.