HepG2 In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

This assay is to provide HepG2-based In Vitro Nitric Oxide Synthases Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HepG2

Human

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Hep G2

Hepatocellular Carcinoma

Oncology

In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

HepG2-cell based In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

Wide ranges of enzyme assays are also available to detect oxidative stress at the cellular level. Enzymes such as NO synthases and xanthine oxidase are known to generate ROS. Enzymes namely superoxide dismutase, catalase and thioredoxin reductase can prevent cells from oxidative damage. GST is another enzyme involved in oxidative stress that catalyzes the reduction of oxidized GSH to GSH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Enzyme Assay