ModifiSite™ Acetylcholinesterase Inhibitor Screening Kit

CAT#: ITS-1125-WB35
Product Type: Assay Kit
Species: All Species
Target: Targets the catalytic activity of acetylcholinesterase (AChE), the primary regulator of synaptic acetylcholine levels.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies acetylcholinesterase (AChE) inhibitor potency using a colorimetric (412 nm) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Acetylcholinesterase Inhibitor Screening Kit provides a rapid, non-radioactive solution for evaluating enzyme inhibition in drug discovery. This high-throughput compatible system utilizes an improved Ellman method to determine percent inhibition, supporting the development of therapeutic candidates for Alzheimer's and other neurological disorders by providing precise data for lead optimization and efficacy testing.
Features
Rapid High-Sensitivity Detection: Offers a linear range of 10-600 U/L, delivering precise results within a 10-minute reaction period at ambient temperatures.
Automated HTS Compatibility: Features a homogeneous "mix-incubate-measure" format, making it ideal for large-scale liquid handling systems and automated screening platforms.
Minimal Sample Requirement: Utilizes as little as 5 µL of test compound per assay, preserving precious libraries while maintaining exceptional reproducibility.
Process Relevance
This kit facilitates the rapid identification of potent leads to streamline hit-to-lead transitions in neuro-pharmacological drug development.
Application Stage
Ideal for early-stage drug discovery, chemical library profiling, and secondary screening for potency and selectivity assessment.
Applications
This assay supports high-throughput screening of chemical libraries, natural product extract evaluation, and kinetic profiling of reversible or irreversible enzyme inhibitors.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Targets the catalytic activity of acetylcholinesterase (AChE), the primary regulator of synaptic acetylcholine levels.
Detection Method
Absorbance at 412 nm
Sample Type
Chemical compounds
Research Areas
Neurodegenerative diseases, toxicology and pesticide research, neuromuscular disorders, cholinergic signaling physiology, etc.
Size
100 tests
Data Output Type
Quantitative measurement of enzyme inhibition percentage and determination of IC50 values via colorimetric absorbance.
Assay Time
30 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Acetylcholinesterase is a critical enzyme at neural synapses and neuromuscular junctions that terminates signaling by hydrolyzing the neurotransmitter acetylcholine. Its dysregulation is central to Alzheimer's pathogenesis, making its inhibition a primary clinical strategy for enhancing cholinergic tone and cognitive function.
Pathways
The enzyme acts as the terminal regulator of the cholinergic synapse pathway, modulating signal duration for nicotinic and muscarinic receptors. Inhibitors prevent acetylcholine breakdown, effectively prolonging postsynaptic stimulation and influencing downstream calcium signaling and neuronal excitability.
For Research Use Only | Not For Clinical Use
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