Custom In Vitro Study Service for Synthetic Circular RNA (circRNA)
Introduction
Facing long drug development cycles, gene expression issues, or novel target validation challenges? Our Custom In Vitro Study for Synthetic Circular RNAs service accelerates drug discovery via advanced RNA engineering and high-throughput screening. At Creative Biolabs, we offer solutions for circRNA research hurdles (expression validation, functional analysis, etc.), delivering reliable data to advance your pipeline toward clinical application.
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Custom In Vitro Study for Synthetic Circular RNAs
Circular RNAs (circRNAs) are a distinct class of non-coding RNAs with a covalently closed loop structure, giving them exceptional stability. Emerging research shows they play key regulatory roles in cellular processes, related to major diseases, and their high abundance and tissue specificity make them promising diagnostic biomarkers and therapeutic agents.
The unique properties of circRNAs make them highly promising for therapeutic applications, but their study requires specialized tools and expertise. Our custom in vitro service is designed to support the entire research and development cycle, from initial design to functional validation.
Design and Synthesis Methods
Fig.1 In vitro experimental and computer simulation methods for the detection and functional analysis of circular RNAs.1
Our process begins with an in-depth bioinformatics analysis to ensure the optimal circRNA sequence and design. We then use state-of-the-art methods for synthetic circRNA production, including proprietary ligation and circularization technologies. This guarantees the generation of high-purity, functional circRNAs with superior stability, which is essential for accurate and reproducible in vitro studies. Our synthesis platform allows for rapid customization and scalability, providing you with the exact RNA molecule you need.
Functional Research and Application
We enable a wide range of functional studies to uncover the biological roles of your circRNA of interest. Our services are applied to:
- Mechanism of Action Studies: Determine how circRNAs function as microRNA sponges or interact with RNA-binding proteins to regulate gene expression.
- Disease Modeling: Use in vitro cell models to investigate the role of circRNAs in pathological processes like inflammation, apoptosis, and fibrosis in heart disease, or cell proliferation and metastasis in cancer.
- Biomarker Validation: Confirm the diagnostic and prognostic potential of specific circRNA candidates identified in clinical samples.
- Therapeutic Development: Validate the efficacy of synthetic circRNAs as a delivery platform for encoding therapeutic proteins or peptides.
Advantages and Characteristics
Our service leverages the inherent advantages of synthetic circRNAs, including their remarkable stability and long half-life, which outperform traditional linear RNA molecules. These characteristics ensure more predictable and sustained effects in your experiments, leading to more conclusive results. By using a controlled, synthetic approach, we eliminate the variability associated with endogenous circRNA expression, allowing you to isolate and study the function of a single target molecule.
Workflow
- Project Consultation & Design: We collaborate to define project scope, objectives, and experimental design. Our scientists design optimal circRNA expression cassettes or knockdown vectors for robust, specific results.
- circRNA Synthesis & Vector Construction: Using proprietary RNA engineering platforms, we synthesize high-purity synthetic circRNAs or construct expression vectors, ensuring stable, efficient in vitro study platforms.
- Functional In Vitro Assays: We conduct assays like real-time PCR, western blotting, cell function tests, and protein-circRNA interaction assays (co-IP/pull-down) to assess circRNA functional impact.
- Data Analysis & Reporting: Our bioinformatics team processes raw data, providing detailed analysis and comprehensive reports with findings summaries, raw data, and publication-ready graphs.
- Deliverable Presentation & Discussion: We present results, offer expert insights, and discuss next research steps with your team.
- Typical Timeframe: 8-12 weeks, depending on design complexity and number of assays.
- Final Deliverables: Comprehensive technical report, raw data files, and final circRNA expression vectors or purified circRNA for your research.
What We Can Offer
At Creative Biolabs, we are not just a service provider; we are your dedicated scientific partner, committed to tailoring our expertise to your unique research needs. Our comprehensive offerings for Custom In Vitro Study for Synthetic Circular RNAs are designed to provide you with the exact support you need, from initial concept to final data.
End-to-end Project Management
We provide a seamless, one-stop service from initial consultation and design to final data delivery and interpretation.
Custom Vector & Construct Design
Our advanced bioinformatics and molecular biology teams will design and synthesize custom circRNA expression or knockdown vectors optimized for your specific target and cell line.
Broad Functional Assay Portfolio
Our services are not limited to a single assay. We offer a diverse range of functional studies, including gene silencing, over-expression, and protein interaction analysis, to fully characterize your circRNA's role.
Versatile Disease Applications
We have extensive experience applying our circRNA expertise to a variety of research areas, including oncology, cardiology, and beyond.
Exceptional Quality Control
Every step of our workflow is supported by rigorous quality-by-design (QbD) principles and advanced analytical techniques to guarantee the purity, stability, and functionality of your circRNA constructs.
Expert Consultation and Support
Our team of experienced scientists is available to provide expert guidance and support throughout your project, ensuring your research is set up for success from day one.
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Customer Reviews
FAQs
What kind of quality control do you perform on your synthetic circRNAs?
We use a multi-step quality control process, including gel electrophoresis to confirm circularization and purity, as well as quantitative RT-PCR to verify expression levels. We ensure that our final product meets the highest standards for your research.
Why should I choose synthetic circRNAs over other types of RNA for my in vitro studies?
Synthetic circRNAs offer several key benefits, primarily their exceptional stability due to the closed-loop structure, which prevents degradation by exonucleases. This ensures more robust and long-lasting effects in your experiments, leading to more reliable data compared to linear RNAs.
I've identified a promising circRNA in my clinical samples, but I don't know its function. Can you help me?
Absolutely. Our service is designed to help you move from identification to functional characterization. We can assist with the entire process, including designing the in vitro study, performing the necessary assays, and helping you interpret the results to uncover the circRNA's biological role.
There are various cyclic methods. How to choose the appropriate one?
The selection should be based on sequence characteristics and experimental requirements: The ribozyme method is suitable for synchronous circular transcription during transcription and is easy to operate; Biological ligation methods (such as T4 RNA ligase) are suitable for in vitro ligation of linear RNA precursors and offer greater flexibility. The team will recommend the best solution based on the project requirements.
At Creative Biolabs, we are committed to being your partner in scientific discovery. Our comprehensive service for Custom In Vitro Study for Synthetic Circular RNAs provides the expertise and technology you need to accelerate your research and translate your discoveries into impactful solutions.
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Reference
- Bibi, Alessia, et al. "Circular RNA regulatory role in pathological cardiac remodelling." British Journal of Pharmacology 182.2 (2025): 316-339. https://doi.org/10.1111/bph.16434. Distributed under Open Access license CC BY 4.0, without modification.
