Membrane proteins serve as the targets of more than 50% modern drugs due to their irreplaceable roles in various physical activities and close relations with many kinds of diseases. As a leading research institution as well as a well-recognized service provider in the field of antibody discovery and development, Creative Biolabs has established a series of premade antibody libraries for de novo discovery of superior anti-membrane protein antibodies. Our ready-to-use premade libraries cover many species (human, mouse, rat, chicken, camel, etc.) and formats (scFv, Fab, VHH), based on non-immune hosts or synthetic approaches. Particularly, now we provide professional services of anti-membrane protein single domain antibody (sdAb) development services using premade library screening.
Single domain antibody (sdAb) represents the smallest antibody fragment that retains high specificity and affinity. They were first identified in camelids as a novel antibody isotype with heavy chain only. sdAbs combine the advantages of conventional antibodies with small molecule drugs. Given to the smaller size (12~15 kDa), sdAbs are able to bind conformational epitopes that are not accessible to larger molecules, such as full-length antibodies (150 kDa) or Fabs (50 kDa). Moreover, they also have better tissue penetration abilities (even through BBB) and are much easier to manufacture. On the other hand, comparing to small molecules, sdAbs retains good specificity, affinity but minimum cytotoxicity as antibodies.
Native premade single domain antibody libraries are constructed from non-immunized camels/llamas. VHH gene pool will be isolated and amplified in vitro, packaged into phage vectors, creating a comprehensive VHH library. Of note, we also have 2 well-established human sdAb libraries that allow rapid discovery of large numbers of high-potency camelized human sdAbs. The HuSdL libraries are generated based on camelized human VH3 in FR2. Diversity was introduced by random mutagenesis in different CDR regions. Meanwhile, human HCDR3 was elongated and sequence-randomized. Therefore, the libraries can raise camelized human antibodies with total human origin, which guarantees low immunogenic potential in humans, especially for long-term and multiple-dose administration.
|Library ID||Library Format||Library Source||Library Capacity|
|HuSdL-1||Camelized synthetic VHH||Human||1.5×109|
|HuSdL-2||Camelized synthetic VHH||Human||2.5×1010|
Highlight advantages of the premade single domain library screening for anti-membrane protein antibody discovery:
Creative Biolabs has extensive experience in raising high-quality anti-membrane protein antibodies to accelerate custom projects. Our ready-to-use premade libraries cover many species (human, mouse, rat, chicken, camel, etc.) and formats (scFv, Fab, VHH), based on non-immune hosts or synthetic approaches. Additionally, integrated membrane protein immunogen preparation service is also available upon request, with multiple options (e.g. liposomes, micelles, nanodiscs, whole cells, etc.). Our technique staff is specialized in dealing with all kinds of GPCR immunogens, ion channel immunogens, and transporter immunogens. Please feel free to contact us for more details.