How do you handle "stutter" peaks in microsatellite analysis?

PCR stutter is a common artifact in STR analysis. Creative Biolabs uses optimized polymerase chemistries to minimize stutter. Furthermore, our bioinformatics pipeline utilizes specific filtering algorithms to mathematically subtract stutter patterns, ensuring that the called peaks represent true biological alleles.

Can you detect Copy-Neutral LOH (CN-LOH)?

Yes. Traditional deletion assays often miss CN-LOH because the copy number remains two. By combining our quantitative allele ratio analysis with specific SNP-based approaches, we can identify regions where heterozygosity is lost despite a normal DNA copy number, a frequent occurrence in hematological malignancies.

Loss of Heterozygosity (LOH) & Allelic Imbalance Analysis Service

Creative Biolabs distinguishes itself as a premier provider of loss of heterozygosity (LOH) and allelic imbalance analysis, furnishing the scientific community with critical genomic insights pivotal to the advancement of oncology and genetic research. This comprehensive service offering - encompassing assay design, rigorous sample processing, and sophisticated bioinformatics - delivers high-fidelity data concerning chromosomal deletions and copy-neutral events. By engaging with Creative Biolabs, researchers secure access to expert molecular characterization that validates cellular models, facilitates biomarker discovery, and elucidates complex genetic mechanisms, thereby optimizing research pipelines and mitigating internal resource constraints.

Unmasking Hidden Allelic Vulnerabilities in Cancer

Loss of heterozygosity is a fundamental genomic aberration where allele loss unmasks recessive tumor suppressor mutations, consistent with Knudson's "Two-Hit Hypothesis." Contemporary research highlights the importance of copy-neutral LOH and "collateral lethality," where concurrent loss of metabolic genes creates specific pharmacological vulnerabilities. Precise characterization of these events is crucial for understanding tumor evolution, validating cellular models, and formulating targeted therapeutic strategies.

What We Can Offer

Customized Marker Panel Design

We design tailored panels using highly polymorphic STR markers and SNPs targeting specific genes. This ensures maximum informativeness for your unique project, delivering precise genetic data relevant to your specific research goals.

Advanced Detection Capabilities

Our high-resolution analysis distinguishes subtle Allelic Imbalance and "invisible" copy-neutral LOH events often missed by standard assays. We provide the sensitivity needed to detect complex chromosomal anomalies, ensuring you capture every critical genomic alteration in your samples.

Challenging Sample Optimization

We utilize proprietary protocols optimized for extracting and amplifying DNA from degraded, archived FFPE blocks. This ensures reliable data recovery from samples stored for over a decade, allowing you to unlock valuable insights from historical tissues.

Quantitative Bioinformatic Analysis

We perform rigorous calculations of allelic imbalance ratios (AIR) using Creative Biolabs' validated algorithms. By filtering PCR stutter artifacts and accurately quantifying mosaicism, we transform raw data into precise, statistically significant biological conclusions for your downstream applications.

Loss of Heterozygosity (LOH) & Allelic Imbalance Analysis Service at Creative Biolabs

Why Choose Us?

Key Advantages	Unique Features
Decades of Genomic Leadership	Standing at the forefront of the industry, we blend extensive historical expertise with modern technological power. This unique combination ensures your project benefits from deep scientific knowledge and the latest advancements in genetic analysis.
Uncovering Hidden Alterations	Our distinct advantage lies in detecting "invisible" copy-neutral LOH events that others miss. We provide the resolution necessary to reveal these critical genomic changes, ensuring comprehensive profiling of your disease models and samples.
FFPE Sample Mastery	We employ specialized protocols specifically designed for degraded FFPE samples, ensuring success where standard methods fail. Our approach recovers high-quality genetic data from archived tissues, allowing you to maximize the value of your biobanks.

Key Advantages

Unique Features

Decades of Genomic Leadership

Standing at the forefront of the industry, we blend extensive historical expertise with modern technological power. This unique combination ensures your project benefits from deep scientific knowledge and the latest advancements in genetic analysis.

Uncovering Hidden Alterations

Our distinct advantage lies in detecting "invisible" copy-neutral LOH events that others miss. We provide the resolution necessary to reveal these critical genomic changes, ensuring comprehensive profiling of your disease models and samples.

FFPE Sample Mastery

We employ specialized protocols specifically designed for degraded FFPE samples, ensuring success where standard methods fail. Our approach recovers high-quality genetic data from archived tissues, allowing you to maximize the value of your biobanks.

To fully understand the Creative Biolabs advantage, we invite you to get a quote today.

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How to Contact Us

Proficient in processing compromised FFPE specimens and delineating copy-neutral events, our scientific team facilitates the translation of raw genomic data into decisive biological insights. Ready to validate your findings? Reach out to our team to design a panel that fits your specific research needs.