Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs is your solutions partner for peptides discovery. Transform your peptide-based projects and experiments using our robust Phage Display TriCo-C9 Cyclic 9-mer Peptide Library. The cyclic 9-mer peptides with trimer codon randomization provide a library capacity of 1.1×1010 to identify highly specific binders. With industry-proven phage display technology, this phage library for peptide selections is specifically optimized to help you overcome the most difficult drug discovery, diagnostics, and more. Partner with us to enable your projects and achieve your goals with ease.
| Library ID | TriCo-C9 | Library Format | Cyclic 9-mer peptide library |
| Display Technology | M13 pIII fusion, phagemid phage display | Randomization | Trimer codon, avoid stop and non-sense codon |
| Vector System | pCDisplay-3M | Tags | His, Myc |
| Library Capacity | 1.1×1010 | Host Strain | E. coli TG1 |
Reliable research outcomes are dependent on high-quality functional antibodies. The TriCo-C9 Library has undergone a comprehensive QC process to make sure that all of the antibodies in the library meet exceptional performance and functional standards. Users will get a high quality resource that provides them with unmatched reliability and consistency throughout the screening projects because of this uncompromising dedication to excellence.
Library Vector Map
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Library Capacity 1.1×1010 Library 1 Capacity (A, B, C): 3.0× 109 Library 2 Capacity (D, E, F): 4.0× 109 Library 2 Capacity (G, H, I): 4.0× 109 |
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Pilot Sequencing Success Rate 94% (47/50 clones) High Diversity 46 unique clones  
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Amino Acid Distribution Frequency  Cysteine amino acid residues are not present in the Tico-C9 fragment, and the distribution frequencies of the other 19 amino acids range from 3.1% to 8.2%, which agrees with the theoretical distribution probability (1/19 = 5.3%). |
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Our TriCo-C9 Peptide Library has been designed and validated with utmost care to deliver maximum quality and diversity. Validation studies reveal >97% functional inserts, devoid of stop codons or frame-shift mutations, ensuring that each and every screen can be performed with the confidence of a high-fidelity, top-notch peptide pool.
Built on a gold-standard true phage display system, we offer a robust platform for isolating high-affinity peptide ligands with unparalleled sensitivity, tailored for the discovery of therapeutic peptides against a wide array of targets.
Explore a vast peptide universe of over 1010 unique variants. This extraordinary diversity significantly amplifies the probability of unearthing novel binders, opening new avenues in drug discovery, functional studies, and diagnostic assay development.
The TriCo-C9 library has been developed with a mind to meet a range of screening applications. The screening process is highly customizable, letting you precisely tune the screening for your biological system of interest.
Compatible with our high-throughput screening platform, the TriCo-C9 allows large-scale, rapid identification of promising candidates, drastically reducing discovery timelines, and accelerating your projects.
The library has been engineered to yield peptide candidates of highest specificity and strong affinity, essential for discovering compounds with minimal off-target interactions and maximized therapeutic potential.
We offer flexible licensing options to suit your specific application needs for the in-licensed library:
| Research License (Academic Use) |
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| Research License (Industry Use) |
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| Commercial License |
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| Epitope Specific Peptide Discovery | Cell-Targeted Peptide Discovery | Disease-Specific Peptide Discovery |
| Particulate Matter Binding Peptide Screening | Soft Matter Binding Peptide Screening |
Creative Biolabs has established an in-house, end-to-end workflow that provides a high degree of flexibility to meet your needs for time savings and effort and delivers peptide candidates of the highest fidelity tailored to your research or therapeutic goals
Send us your antigen details (recombinant protein, peptide, small molecule, cells, etc.) for initial screening.
Multi-round biopanning with customized protocol and optimized conditions will be carried out to ensure that high-affinity binders are selected.
Detailed report with peptide sequences and binding information will be delivered along with clone information.
Our team of experts is available to assist you in validating and optimizing your antibody candidates for your final application, be it therapeutic or diagnostic.
| Discovery of Binding Peptides for Two Similar Target Proteins | ||
| Creative Biolabs has developed the experience and knowledge to identify highly specific binding peptides for even the most challenging targets, including when two very similar proteins are involved. Our client was interested in discovering peptide binders that could be specific to two similar target proteins, T-A and T-B, in one go, a necessary step for their therapeutic development. | ||
| Screening Strategy | An intensive 4-round alternative screening was carried out with the Trico-9 Peptide Library with biopanning performed alternatively between target T1-A and T1-B. This approach was designed to increase the odds of finding peptides with an affinity to both targets. | |
| Key Results |
  Throughout the screening process, we observed enriching effects against both target proteins. This was further corroborated by polyclonal phage ELISA. |
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2. Identification of Dual Binders Through monoclonal phage ELISA, we successfully identified 40 out of 80 clones that are positively bound to both T-A and T-B. Subsequent DNA sequencing of these positive clones revealed 7 unique peptide sequences, highlighting the diversity of the identified binders. |
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3. Peptide Synthesis & Validation The identified unique peptide sequences were then synthesized in both cyclic and linear forms, all achieving a desired purity of over 95%. We proceeded to validate their binding activities: 
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Our comprehensive workflow, from alternative phage display screening and rigorous validation to high-purity peptide synthesis and functional assessment, underscores our capability to tackle complex binding challenges and provide high-quality, actionable results for our clients.
Creative Biolabs offers a range of other premade peptide libraries designed to meet diverse research requirements. These include 16-mer and 20-mer linear peptide libraries and Protease-10A peptide library, ideal for discovering high-affinity binders across a wide variety of targets. For more challenging targets and specialized projects, we also provide the latest C9 Expansion Library, designed for high-throughput screening of difficult or complex targets. Whether you are looking to license out our libraries or utilize our comprehensive screening services, we are equipped to help you identify the best peptide binders for your specific application.
| Library ID | Display Technology | Library Format | Library Size | Out-licensing |
| TriCo-16 | pIII-fusion, True Phage Display | 16-mer peptide library | 1.1×1010 | ✔️ |
| TriCo-20 | pIII-fusion, True Phage Display | 20-mer peptide library | 1.3×1010 | ✔️ |
| TriCo-C9 | pIII-fusion, Phagemid Phage Display | Cyclic 9-mer peptide library | 1.1×1010 | ✔️ |
| TriCo-C9L | pIII-fusion, Phagemid Phage Display | Cyclic 9-mer peptide library | 6.8×1010 | Screening-only |
| Protease-10A | pIII-fusion, Phagemid Phage Display | HiAffi tagged 10 mer peptide library | 1.1×1010 | ✔️ |
Our cutting-edge platform delivers rapid, high-impact results, accelerating your research from concept to clinical application.
Partner with our highly experienced team for comprehensive support and insightful guidance, ensuring a smooth and successful project journey.
We offer bespoke screening strategies meticulously designed to meet your specific target requirements, maximizing the relevance and efficacy of your findings.
Rely on our robust and validated systems for consistently reproducible and scalable results, providing the dependable data foundation your critical projects demand.
Contact us for more information on how our libraries and screening platforms can help you achieve your research goals!
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
