FcR Binding Assay

The Fc-gamma receptor (FcR), a member of the immunoglobulin superfamily, is divided into FcγRI (CD64), FcγRIIa (CD32a), FcγRIIb (CD32b), FcγRIIIa (CD16a) and FcγRIIIb (CD16b). Historically, these receptors have been classified based on their affinity for specific IgG subclasses and whether their binding initiates activation or transmits inhibition signals. The Fc-gamma receptor is expressed on many immune cells and binds to monomeric IgG with high affinity, thereby modulating the immune response.

Measuring the affinity of antibodies for Fc receptors has become an integral part of biotherapeutic drug development. Creative Biolabs provides off-the-shelf FcR binding assays based on our real-time kinetic analysis, flow cytometry and surface plasmon resonance (SPR) technology platforms. FcR binding assay is used to support the process development of therapeutic antibodies, such as to evaluate different clones, promote different production processes, or prepare materials for different formulations. These Fc receptor binding assays will enhance the overall understanding and demonstrate comparability in the development of therapeutic antibodies.

Binding Assay Development for Your Custom Antibody

Creative Biolabs uses a versatile label-free system with excellent sensitivity for FcγR binding analysis of various IgG antibody isotypes or Fc fusion proteins. Our team of scientists works with you to optimize the performance of FcγR binding assays and apply the experience to new antibody therapies, which can be analyzed in many different formats to meet your needs. Example assays include:

• FcγR1/ CD64
• FcγR2a/ CD32a R variant
• FcγR2a/ CD32a H variant
• FcγR2b/CD32b

• FcγR3a/ CD16a V variant
• FcγR3a/ CD16a F variant
• FcγR3b/ CD16B

With our state-of-the-art technology and experienced scientists, Creative Biolabs has introduced a novel, reliable and highly flexible platform for evaluating FcγR binding properties. We can perform FcyR binding assays based on the following techniques:

• Conventional ELISA
• Flow cytometry
• Surface Plasmon Resonance (SPR) / Octet Biolayer Interferometry (BLI) (recommended): real-time unlabeled full kinetic analysis, and reporting of affinity constants (KD) associated with detailed or "on" rates (Ka) and dissociation rates (Kd) or "closed" rates.

For more details, please feel free to contact us or send us a query directly.

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