Decoding Immune Complexity for Breakthrough Therapies

In the intricate landscape of immunology, understanding the functional capacity of immune cells is pivotal for advancing therapeutic strategies and disease management. Immune cells such as T cells, B cells, and natural killer (NK) cells play crucial roles in maintaining the body's defense mechanisms. However, their functional dynamics often remain a mystery, particularly in complex disease states. Creative Biolabs introduces our immune cells function assay, a comprehensive service designed to elucidate the capabilities of various immune cell subsets, providing critical insights into immune responses and aiding in the development of next-generation immunotherapies.

Service Overview

Creative Biolabs' immune cells function assay is a comprehensive platform that encompasses multiple specialized services to provide a thorough analysis of immune cell functionality:

Technologies

The immune cells function assay utilizes a variety of advanced techniques, including:

• Flow cytometry: For detailed analysis of cell surface markers, intracellular proteins, and cytokine production, allowing for the precise quantification of immune cell subpopulations and their functional states.
• ELISA: Used to quantify the levels of cytokines, chemokines, and other soluble factors secreted by immune cells during activation.
• Proliferation assays: Utilizing [³H]-thymidine incorporation or other staining methods to assess the proliferative capacity of immune cells in response to specific antigens or stimuli. Cytotoxicity Assays: Evaluating the ability of NK cells and cytotoxic T lymphocytes (CTLs) to kill target cells, typically using assays like chromium release, LDH release, or flow cytometry-based methods. T Cell Activation Assays: Monitoring the activation and expansion of T cells upon exposure to antigens, using techniques like intracellular cytokine staining (ICS) and CD69/CD25 expression analysis.

Fig.1 Reciprocal interactions between different immune cell types and the tumor vasculature in the tumor microenvironment.¹

We also offer a wide range of other in vitro immune cell function assays to support diverse research needs. Each assay is designed to provide detailed insights into immune cell behavior, enabling a deeper understanding of immune responses and therapeutic effects.

• T Cell Targets and Activation
  • T cell target and activation
  • T cell proliferation
  • Target expression profiling on T cell subsets
  • Screening assay to enhance proliferation and survival (MLR)
  • Regulation of differentiation and cytokine profiles
  • Immune cell migration assay
  • Antigen-specific recall response assay
  • Screening for the ability to reverse T-cell exhaustion
  • Suppression assay using regulatory T cells
  • Mixed lymphocyte reaction (PBMC / T cell / DC, one-way or two-way)
  • Immune checkpoint blocking assay
  • Cytokine secretion profile (ELISA, Luminex, ELISpot)
  • T cell surface marker expression analysis (flow cytometry)
• Macrophage and Dendritic Cell Targets
  • Analysis of target expressions on subsets (still and active)
  • Screening for M1 / M2 / MDSC regulation
  • Screening for regulation of tumor conditional responses (phenotype and cytokine/chemokine production)
  • Effect on antigen presentation
  • Antibody-dependent cellular phagocytosis (ADCP)
• NK cell target
  • Target expression on NK cells
  • Regulate activation and kill
  • Antibody-dependent cellular cytotoxicity (ADCC)
• B Cell Target
  • Target expression profiling on B cell subsets (rest and activation)
  • Regulatory antibody production
  • Regulate antigen-presenting cell function and cytokine release
  • BCR signaling (BTK, CARD11 / BCL10 / MALT1 complex)
• Mast Cells and Basophils
  • Degranulation and histamine release
  • Cell migration and chemotaxis

Frequently Asked Questions

Q1: What sample types can be used for the Immune Cells Function Assay?

A1: We accommodate a wide range of sample types, such as whole blood, peripheral blood mononuclear cells (PBMCs), tissue biopsies, and isolated immune cell subsets.

Q2: How do you guarantee the accuracy and reliability of your assays?

A2: Our assays are conducted using state-of-the-art technology and are validated against industry standards. Each assay undergoes rigorous quality control, and results are carefully analyzed by our expert team to ensure data accuracy and reproducibility.

Q3: Can the assays be customized to focus on specific immune cell subsets or cytokines?

A3: Absolutely. We offer customizable options to target specific immune cell populations or cytokines based on your research needs. Our team will work closely with you to design an assay that aligns with your study objectives.

Q4: How long does it typically take to receive results from the Immune Cells Function Assay?

A4: The turnaround time may vary based on the assay's complexity and the specific services requested. We can provide more precise timelines upon discussion of your project requirements.

Q5: What kind of data analysis is provided with the service?

A5: We provide comprehensive data analysis, including detailed reports with statistical analysis, graphical representation of results, and expert interpretation. If needed, we can also offer follow-up consultations to discuss the findings and their implications for your research.

At Creative Biolabs, we understand that the intricacies of immune cell function are pivotal in driving the next wave of immunotherapies. Our immune cells function assay is meticulously designed to provide you with the comprehensive and actionable insights needed to push your research forward. Whether you're exploring immune memory, evaluating T cell activation, or analyzing leukocyte dynamics, our team is here to support your journey. Contact us today to discuss how we can tailor our services to meet the specific needs of your project, and together, let's unlock the full potential of your immune-based research.

Reference

1. Stockmann, Christian, et al. "The impact of the immune system on tumor: angiogenesis and vascular remodeling." Frontiers in oncology 4 (2014): 69. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use