TCR-T and CAR-T NK/MA Cell Activation and Expansion

The research and development of cellular immune therapy for a variety of diseases is the main business of Creative Biolabs, including the international leading CAR-T design construction cell therapy and TAR-T design construction cell therapy, Creative Biolabs offers programming CAR technologies and transfection technologies for you to chose, our targets cover blood tumors entity related diseases such as cancer and virus infection, we are committed to establishing the domestic and foreign leading technology research and development platform.

TCR-T and CAR-T Cell Separate Acquisition Platform

Several steps are required for the production of CAR-T or TCR-T T cells. We are able to conduct quality control test throughout the entire protocol.

Fig.1 Cellular therapy has several pathways to the patient.

TCR-T and CAR-T T Cell Activation and Expansion Service Overview

• We used specific antibody-bead conjugates or markers to isolate T-cell subsets at CD4 or CD8 component levels
  The T cell receptor (TCR) on CD4+ adjuvant T cells and CD8+ cytotoxic T cells binds to the antigen on the APC surface by MHC complex structure. This triggers the initial activation of T cells.

• Easily provide an anti-CD3 or anti-CD28 monoclonal antibody, which is an effective drug to activate T cells

  • T cells encounter dendritic cells (DC) with homologous peptides in MHC molecules and bind to peptide-MHC through CD3 and CD4 or 8. Subsequently, CD86, CD80, OX40L and 4-1bbl were co-stimulated by DC binding, thus induces full activation of T cells.
  • CD28 on T cells binds to one of two molecules on APC B7.1 (CD80) or B7.2 (CD86) to initiate T cell proliferation.
• Our Anti-CD3 beads, either alone or in combination with donor cells and growth factors such as IL-2, induce rapid growth of T cells
• Culture conditions are further refined to polarize T cells to a specific phenotype
  WAVE Bioreactor, G-Rex and CliniMACS Prodigy are bioreactor culture systems which can be used to culture CAR-T cells more efficiently and safely
• The affinity of cell activation
  Our instructed team is able to maximize T cell activation, which enables T cell amplification by combining CAR with higher affinity.

CAR-NK Cell Activation and Expansion Service Overview

We offer IL-2 and IL-15 cytokine that activate NK cells, which leads to activation of JAK/STAT, PI3K, MAPK, and NF-κB pathways.

✔  IL-2 stimulates cellular proliferation and enhances cytotoxicity

✔  IL-15 significantly improves NK cell survival although it only stimulates minimal expansion

Fig.2 Cytokine activated NK cells.

CAR-MA Cell Activation and Expansion Service Overview

Macrophages have a unique ability to penetrate solid tumors, while T cells and other immune cells are rejected or inactivated.

✔  CD47 is a negative regulator of phagocytosis, figuring that shifting the balance in favor of macrophage activation and engulfment is a promising therapeutic avenue.

✔  CARPMegf10 initiates engulfment through a localized signaling cascade involving tyrosine phosphorylation.

✔  Megf10 and FcRV robustly trigger engulfment independently of their native extracellular domain.

✔  CD3z of intracellular signaling domains activate engulfment.

Highlights

• Able to develop cellular immunotherapy for a variety of diseases.
• Provide domestic and foreign leading technology research and development platform.
• With scientific and rigorous management, our senior experts will assist you with the experimental project and various technical plan

Please feel free to contact us and our team will contact you as soon as possible.

References

1. Barrett D M; et al. Chimeric Antigen Receptor and TCR-Modified T Cells Enter Main Street and Wall Street[J]. The Journal of Immunology . 2015, 195(3): 755-761.
2. Domogala A; et al . Natural Killer Cell Immunotherapy: From Bench to Bedside[J]. Frontiers in Immunology . 2015, 6: 264.

For Research Use Only | Not For Clinical Use