CAR Design Construction and Plasmid GLP Production

Creative Biolabs develops and designs chimeric antigen receptors (CAR), to detect and fight cancer cells. This protein structure can stimulate cancer-fighting T cells and thus strengthen the patient's immune system. Relies heavily on historical experience and trial, Creative Biolabs accumulates knowledge of T-cell biology, CAR signaling, and rational protein engineering, that will assist you to predict CAR performance in the clinic and research.

CAR Designs Overview

Creative Biolabs provides four types of CAR for experimental research, making your research on cellular gene therapy more effective and convenient.

√  1^st-generation CAR

It contains CD3ζ-chain and the primary transmitter of signals from endogenous TCRs, which show success in pre-clinical trials and entered Phase I clinical trials in ovarian cancer, neuroblastoma and various types of leukemia and lymphoma.

√  2^nd -generation CAR

The co-stimulatory domain is derived from either CD28 or 4-1BB, which are co-stimulatory receptors expressed on the surface of T cells. And the CD28-based CARs show rapid proliferative response and boost effector functions. Meanwhile, the 4-1BB-based CARs manifest a more progressive T cell accumulation.

√  3^rd-generation CAR

Incorporate two co-stimulatory domains, such as CD3 ζ-CD28-41BB, CD3 ζ-CD28-OX40, both are derived from different co-stimulatory proteins, which can enhance further activation signals, proliferation, production of cytokines and effective function.

√  4^th-generation CAR

The CAR is designed to shape the tumor environment by the inducible release of transgenic immune modifiers, such as IL-12, which augments T-cell activation, attracts and activates innate immune cells to eliminate antigen-negative cancer cells in the targeted lesion.

Fig.1 Outline of single-cell sequencing applied to interrogate CAR-T cell biology in preclinical studies.¹

Service Overview

Creative Biolabs is committed to providing a variety of CAR design and construction services to meet the demands and expectations of customers, including but not limited to the following:

Plasmid GLP Production

• Our researchers devoted significant time and effort recording the details of each experimental step and result to ensure GLP-level service.
• We set up GLP laboratory for customers.
• High-quality services will pave the way for its downstream applications such as in vivo testing, DNA antibody production, gene therapy research and so on.

In order to realize downstream applications of preclinical plasmids, Creative Biolabs launches the GLP plasmid preparation service. Each step of plasmid preparation service has been standardized to ensure the consistency of experimental results.

Please feel free to contact us and our team will get back to you as soon as possible.

Reference

1. Tang, Lu, et al. "Insights gained from single-cell analysis of chimeric antigen receptor T-cell immunotherapy in cancer." Military Medical Research 10.1 (2023): 52.

For Research Use Only | Not For Clinical Use